The aim of this study is to determine the role of placental leucine aminopeptidase (P-LAP) in miscarriage and to exclude direct or indirect effects of abortion by searching for the gene for this enzyme in miscarriage. (Qiagen) Total RNA is extracted from fresh placental tissue samples using the QIAamp® RNA Tissue Mini Kit methodology. Samples can be viewed using a 0.7% Agrose gel. After RNA extraction, reverse transcribe the total RNA sample using RT-PCR (QIAGEN One Step RT-PCR Kit). Amplify the RT reaction sample (1 ml) using RT-PCR. PCR products (10 µl) per lane are separated by electrophoresis on a 2.0% agarose gel. During abortion, P-LAP may play a role in delaying trophoblast invasion. Collected data show that a woman undergoing a normal pregnancy (28±0.45) has her P-LAP mRNA expression in the placenta and reduced miscarriage. There is also a difference between one miscarriage and repeated miscarriages. The mean values for single miscarriages (1st and 2nd) were (16.1% ± 0.84) and (13% ± 0.24), respectively, and for repeated miscarriages (1st and 2nd) were (12% ± 0.19). is. and (9% ± 0.52). P-LAP expression is significantly lower in miscarriages (P = 0.05) and significantly lower in recurrent miscarriages than single miscarriages. The fact that there is less P-LAP mRNA in aborted placentas may suggest that p-LAP function is an important component of pregnancy management.
Author(s) Details:
Taghreed H. AL Sadoon,
Department of Biochemistry, College of Medicine, University of Tikrit, Iraq.
Ferah Gh. Al-Salihi,
Department of Chemistry, College of Sciences, University of Tikrit, Iraq.
Adnan I. Al-Badran,
Department of Biology, Cell and Biotechnology Research Unit, College of Sciences, University of Basrah, Iraq.
Please see the link here: https://stm.bookpi.org/CIMMS-V2/article/view/8222
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