Flavonol Glycosides from the Aerial Parts of Indigofera hirsuta and Anti-inflammatory Activity of n-butanol Fraction | Chapter 12 | Technological Innovation in Pharmaceutical Research Vol. 2

Aims : The aim of this study was to investigate the phytochemical properties of the n-butanol soluble fraction of Indigofera hirsuta aerial parts and to assess the fraction's anti-inflammatory behaviour using laboratory animal models.

Study Design: Isolation and elucidation of bioactive substances, as well as anti-inflammatory activity testing on the soluble fraction of n-butanol.

Place and Duration of Study:  Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria, Nigeria, and period of study: Between January and October of 2011, the research was done.

Methodology: Various spectroscopic techniques were used to identify the compounds isolated. In a rat model, the influence of the n-butanol fraction on carrageenan-induced oedema was investigated.

Results : Two flavonol glycosides, Kaempferol-3-O—D-glucopyranoside (T2) and Kaempferol-7-O—D-glucopyranoside (T3), were isolated (Q3). At doses of 150 and 300 mg/kg measured, the fraction significantly (P =.05) prevented carrageenan-induced paw oedema. The highest dose tested (300 mg/kg) had a higher percentage anti-inflammatory effect at the peak hour than ketoprofen (10 mg/kg), the normal anti-inflammatory agent.

Conclusion: This study's findings indicate that the n-butanol soluble fraction of Indigofera hirsuta aerial parts contains anti-inflammatory bioactive compounds.

Author (s) Details

L. S. Abbas
Department of Medical Laboratory, Hospital Services Management Board, Gusau, Nigeria and Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria, Nigeria.

M. I. Abdullahi
Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria.

M. G. Magaji
Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria, Nigeria.

M. I. Sule
Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University, Zaria, Nigeria.

B. Bawa
National Agency for Food, Drugs Administration and Control (NAFDAC), Nigeria.

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