Determination of the Area and Number of Pulmonary Alveoli through the Normal aging Process in CD1 Mouse | Chapter 9 | Research Aspects in Biological Science Vol. 3

The ageing process produces structural changes as well as a decrease in function in the lungs. Depending on the species and strain, several pulmonary parameters alter. The CD1 mouse strain has been frequently utilised to study many elements of the ageing process. The purpose of this research was to assess the size and quantity of lung alveoli in CD1 mice as they grew older. A light microscope was used to analyse hematoxylin and eosin-stained paraffin-embedded slices of lungs from CD1 mice aged 2, 6, 12, 18, or 24 months. Images were obtained using a camera attached to image analysis software to measure regions and count alveoli. The alveolar area differed significantly between the ages studied (F=87.53, Sig.=0.000). The alveolar area of 6-, 12-, 18-, and 24-month-old mice was considerably bigger than that of 2-month-old mice (all p values 0.001). In addition, the alveolar number differed substantially across the ages studied (F=3.21, Sig.=0.023). When compared to 6-, 12-, and 18-month-old mice, the number of alveoli in 2-month-old mice was significantly higher than in the other age groups (p values of 0.044, 0.014, and 0.002, respectively). We detected an increase in alveolar area and a decrease in alveolar number as a result of the ageing process. This insight may aid researchers in better understanding the pathologic changes that explain the vulnerability of elderly persons to chronic lower respiratory tract illnesses.

Author(s) Details:

Gilberto Jaramillo-Rangel,
Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon, Mexico.

Esthefania Gutiérrez-Arenas,
School of Biology, Autonomous University of Sinaloa, Culiacan, Sinaloa, Mexico.

Adriana Ancer-Arellano,
Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon, Mexico.

María-de-Lourdes Chávez-Briones,
Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon, Mexico.

Ricardo M. Cerda-Flores,
School of Nursing, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon, Mexico.

Marta Ortega-Martínez,
Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, Nuevo Leon, Mexico.

Please see the link here: https://stm.bookpi.org/RABS-V3/article/view/7051