The goal of this study was to see if there was any genetic variation among different pearl millet germplasm lines using a combination of drought-related morpho-physiological characteristics and SSR molecular markers. Study Design: 96 pearl millet germplasm lines were tested for drought resistance utilising morphological and physiological parameters as well as SSR markers in this study. The current study was carried out from July 2019 to December 2020 at the College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India. The study's goal was to document several morphological and physiological features associated with drought resistance and susceptibility. In the lab, 35 microsatellite markers were utilised to examine the variability among the pearl millet genotypes under investigation. The genotypes of pearl millet were classified based on their morpho-physiological properties. Twenty-two of the 35 SSR markers were effectively amplified across all germplasm lines, with seven SSR markers being polymorphic and fifteen being monomorphic. As a result, all seven polymorphic SSR markers were employed to amplify all 96 germplasm lines. PIC values ranged from 0.0939 to 0.2980, with an average of 0.2274. The markers Xibmsp26 and Xibmsp29 (0.2980) had the highest PIC value, followed by Xibmsp03 (0.2392), Xibmsp29 (0.2392), Xibmsp06 (0.2289), and Xibmsp07 (0.1948). The marker Xibmsp01 had the lowest PIC value (0.0939). The average major allele frequency value was 0.8363, with a range of 0.7604 to 0.9479. The genetic diversity value ranged from 0.0987 to 0.3644, with a mean of 0.2665. Conclusions: A total of 22 pearl millet genotypes were discovered to be clustered distantly from the rest of the genotypes based on morpho-physiological data. These genotypes may be drought tolerant since they are linked to drought tolerant morpho-physiological features, although the majority of the genotypes were found to be drought vulnerable.
Author (S) Details
M. L. Choudhary
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.
M. K. Tripathi
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.
Sushma Tiwari
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.
R. K. Pandya
Department of Plant Pathology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.
Neha Gupta
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.
Niraj Tripathi
Directorate of Research Services, JN Agricultural University, Jabalpur 482004, India.
Prerana Parihar
Department of Plant Pathology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.
View Book :- https://stm.bookpi.org/NVST-V5/article/view/4100
No comments:
Post a Comment