Morpho-physiological and Molecular Characterization of Pearl Millet [Pennisetum glaucum (L.) R. Br.] Germplam Lines for Drought Tolerance | New Visions in Science and Technology Vol. 5

 The goal of this study was to see if there was any genetic variation among different pearl millet germplasm lines using a combination of drought-related morpho-physiological characteristics and SSR molecular markers. Study Design: 96 pearl millet germplasm lines were tested for drought resistance utilising morphological and physiological parameters as well as SSR markers in this study. The current study was carried out from July 2019 to December 2020 at the College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India. The study's goal was to document several morphological and physiological features associated with drought resistance and susceptibility. In the lab, 35 microsatellite markers were utilised to examine the variability among the pearl millet genotypes under investigation. The genotypes of pearl millet were classified based on their morpho-physiological properties. Twenty-two of the 35 SSR markers were effectively amplified across all germplasm lines, with seven SSR markers being polymorphic and fifteen being monomorphic. As a result, all seven polymorphic SSR markers were employed to amplify all 96 germplasm lines. PIC values ranged from 0.0939 to 0.2980, with an average of 0.2274. The markers Xibmsp26 and Xibmsp29 (0.2980) had the highest PIC value, followed by Xibmsp03 (0.2392), Xibmsp29 (0.2392), Xibmsp06 (0.2289), and Xibmsp07 (0.1948). The marker Xibmsp01 had the lowest PIC value (0.0939). The average major allele frequency value was 0.8363, with a range of 0.7604 to 0.9479. The genetic diversity value ranged from 0.0987 to 0.3644, with a mean of 0.2665. Conclusions: A total of 22 pearl millet genotypes were discovered to be clustered distantly from the rest of the genotypes based on morpho-physiological data. These genotypes may be drought tolerant since they are linked to drought tolerant morpho-physiological features, although the majority of the genotypes were found to be drought vulnerable.

Author (S) Details

M. L. Choudhary

Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.

M. K. Tripathi

Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.

Sushma Tiwari

Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.

R. K. Pandya

Department of Plant Pathology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.

Neha Gupta

Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.

Niraj Tripathi

Directorate of Research Services, JN Agricultural University, Jabalpur 482004, India.

Prerana Parihar

Department of Plant Pathology, College of Agriculture, RVS Agricultural University, Gwalior, 474002 (M.P.), India.

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The Use of Metadiscourse Markers in English Research Articles by Native English Writers vs. Non-Native Writers: A Comparative Analysis | Chapter 2 | Modern Perspectives in Language, Literature and Education Vol. 6

 Metadiscourse markers are thought to be useful for conveying ideas to the audience, producing and organising persuasive writing based on individual expectations and norms, communicating effectively with readers, signalling the speaker's or writer's attitude, engaging the audience, and organising the discourse. The employment of metadiscourse indicators in English research articles by native English writers and non-native English researchers was compared in this study. The metadiscourse paradigm recognised interactive and interactional cues as metadiscourse markers. The researcher attentively examined each of the forty (40) English applied linguistic research publications and looked for interactional indicators in the introduction, material and methods, result and discussion, and conclusion sections. Arabic Journals of Humanities and International Academic Journals provided the articles. An independent sample two-tailed t-test was used to analyse the data. The results demonstrated that native English authors use interactive and interactional metadiscourse markers differently from non-native English writers. Non-native English writers should master the use of metadiscourse devices to write appealing and high-quality research articles, convey ideas to the audience, produce and organise persuasive writing based on the expectations and norms of the individuals involved, communicate effectively to readers, signal the speaker's attitude, engage the audience, and organise the discourse resea

Author (s) Details

Mahmoud Mahmoud
Nizwa University, Oman.

Bilal Zakarneh
Ajman University, UAE.

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