Studies on Bacterial Populations Concomitant with Sclerotium rolfsii sclerotia in Flooded Soil, as Estimated by 16S rRNA Gene, PCR-DGGE and Sequence Analyses | Chapter 08 | New Visions in Biological Science Vol. 4

 The bacterial populations associated with sclerotia of Sclerotium rolfsii, the causal agent of soybean stem rot, were investigated using PCR-DGGE.

Methodology and Results: In a greenhouse and in the field, fungal sclerotia were buried in soil supplemented with organic matter and incubated under flooded circumstances for 15 or 30 days. The viability of the recovered sclerotia, as well as the accompanying bacterial populations, were studied. DGGE band patterns exhibited the most bacterial diversity in samples from soil supplemented with rice straw or wheat bran and inundated for 30 days. The diversity index from organic amendment under 30 day flooding in the greenhouse, for example, was greater than 1.80, whereas the index for other treatments was less than 1.5. This pattern was seen in the field experiment as well. Sclerotial viability and illness incidence were negatively correlated with the diversity index generated from DGGE band patterns. The nucleotide sequences of the DGGE bands revealed that Clostridiaceae members were prominent in samples that had been inundated for 30 days, while Oxalobacteraceae, Nocardiaceae, and Actinomycetaceae were dominant in samples that had not been flooded.
Conclusion: Under flooded conditions, this is the first description of a soil bacterial flora associated with S. rolfsii sclerotia.

Author(S) Details

Appolinaire Adandonon
School of Crop and Seed Production and Management (EGPVS), National University of Agriculture (UNA) of Porto-Novo, Republic of Benin; 08 BP 1055 Cotonou, Republic of Benin.

Noriaki Momma
Department of Research & Development, Institute for Horticultural Plant Breeding, 2-5-1 Kamishiki, Matsudo, Chiba, 270-2221, Japan..

Yuko Takada Hoshino
Division of Environmental Biofunction, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba 305- 8604, Ibaraki, Japan.

Tomoyuki Makino
Division of Environmental Biofunction, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba 305- 8604, Ibaraki, Japan.

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