Cancer is a major cause of death worldwide and angiogenesis
is critical in cancer progression. The development of new blood vessels and the
nutrition of tumor cells are heavily dependent on angiogenesis. Angiogenesis is
the formation of new blood vessels from existing vasculature. In normal
physiology, angiogenesis has a role in embryogenesis, the female reproductive
cycle, and wound healing and bone formation [1]. There are several “on” and
“off” switches that regulate the process. Decreasing or inhibiting angiogenesis
can be therapeutic in cancer and other diseases. Thus, angiogenesis is an
important process that occurs both during health and disease. This study was
conducted to investigate the anti-angiogenic activity of Carica papaya leaf.
Fresh green papaya leaves, were randomly collected from Yadav Nursery, Geeta
Colony, East Delhi. The anti-angiogenic activity of papaya leaf was evaluated
using the docking behaviour of known bioactive compounds of a leaf as ligands
with angiogenic receptors VEGFR1 and VEGFR2 along with their putative binding
sites using Swiss Dock Web server (In silico) and further based on docking
results leaf aqueous extract was used for implantation in the chorioallantoic
membrane (CAM) egg yolk angiogenesis model (in vivo). Docking studies and
binding free energy calculations revealed that among known bioactive compounds
of leaf Ascorbic acid, quercetin, riboflavin and lycopene have maximum free
energy as compared to other investigated ligands. Similarly, CAM assay also
showed the inhibitory effect of the Carica papaya leaf with respect to their
reduction in length, size and junctions of blood capillaries compared to
untreated egg yolk. The results showed that Ascorbic Acid, Quercetin,
Riboflavin and, Lycopene (leaf compounds) can attenuate angiogenesis in
pathological conditions and can be potent in drug discovery as well as medical
science. Further studies need to be conducted to determine anti-angiogenic
pathways followed by leaf extract or juice. It can be concluded that administration
of Carica papaya leaf juice is safe and does induce anti angiogenic process
during disease condition like Cancer.
Author(s) Details
Nidhi Tayal
Centre for Medical Biotechnology, Amity Institute of
Biotechnology, Amity University, Sector-125, Noida - 201 313, India.
Priyansh Srivastava
Centre for Medical Biotechnology, Amity Institute of
Biotechnology, Amity University, Sector-125, Noida - 201 313, India.
Nidhi Srivastava
Centre for Medical Biotechnology, Amity Institute of
Biotechnology, Amity University, Sector-125, Noida - 201 313, India.
Please see the link:- https://doi.org/10.9734/bpi/rpmab/v4/353
No comments:
Post a Comment