Characterization of Cassava Mosaic Disease for Identification and Analysis of Cassava Genotype TME3 Bacteria Artificial Chromosome Libraries| Chapter 7 | Innovations in Microbiology and Biotechnology Vol. 8

 In the study, attempts were made to assemble sequences and label genes residing in the CMD2 position. In sub-Saharan Africa, cassava is a significant crop commercially, still the infection accompanying cassava mosaic disease (CMD) limits the crop's output potential. Traditional genetics and biotechnology are being used to combat the sickness and ensure farmer harvests. The CMD2 opposition locus was mapped in West African genotypes and displayed to confer approximate resistance to all species of CMGs. It is adjoined by three simple series repeats (SSR) markers and one series characteristic amplified region (SCAR) tombstone. However, gene(s) guide the CMD2 locus and their mode of conduct remains obscure. In an effort to discover deoxyribonucleic acid(s) located in CMD2 position region, TME3 BAC collections were secluded for the presence of CMD2 flanking flags. CMD susceptible and opposing cassava genotypes were found to contain 100% of the stones flanking CMD2 position. SNPs and nucleotide deletions were identified within the tombstone sequences but there was no evidence of trait and gravestone association. All the SSR stones flanking CMD2, and the more recently typified CMD3 loci search out be located on chromosome 12. Through BAC pools study hybridization accompanying marker probes, 130 BACs were identified, but only 23 BACs held at least CMD2 specific two gravestones. Whole BAC sequencing identified five clones that plan to the marker regions. BAC29 massed into a 100 kb contig and encoded group repeats of three full length R genes (3.5 kb) and two biased repeats. These R genes were conserved and highly signified in CMD susceptible and CMD resistant cassava genotypes. Promoter sequences came from R genes showed similar temporary expression of GUS as 35S supporter. On cassava genome V6.1 BAC29 sequences were mapped to chromosome 16, removing their potential role in CMD opposing. Efforts should therefore devote effort to something developing polymorphic microscopic markers carefully linked with the CMD2 position that can easily be took advantage of to screen for CMD resistance. In addition, there is need for whole genome sequencing of CMD2 type cassava to label sequences coding for genes hid in CMD2 region.

 Author(s) Details:

Paul Kuria,
Kenya Agricultural Research Organization, PO Box-57811-00200, Nairobi, Kenya.

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