Electron tomography of organic samples is used to study the organization and the structure of all cell and subcellular complexes. We reach isotropic resolution with a mathematical reconstruction method, subsequent maximum a posteriori expectation growth (sMAP-EM), to resolve missing projections in broadcast electron microscopy over incomplete tilts of tomography dossier. Evaluated by the symmetricity of gold atoms, an ellipsoid fitting-based method was grown to realize the quantitative measures of extension and contrast in an automated and objective way in bearing isotropic resolution. Moreover, quantitative estimates were performed comparatively accompanying reconstructions made with widely-used burden back projection and simultaneous repetitive reconstruction method methods. We improved the contrast percentage, enhancing the applicability of further mechanical and semi-automatic reasoning. The method statistically evaluates the sub-capacities containing gold pieces randomly located in miscellaneous parts of the whole volume, accordingly indicating the robustness of the capacity improvement. The improved 3D rebuilding by sMAP-EM enables an study of subcellular structures with embellished three-dimensional resolution and contrast to common methods.
Author(s) Details:
Lassi Paavolainen,
Department
of Molecular and Cellular Biology, University of California Davis, Davis,
California, United States of America and FiMM, University of Helsinki,
Helsinki, Finland.
Pan
Soonsawad,
Department
of Molecular and Cellular Biology, University of California Davis, Davis,
California, United States of America and Department of Anatomy, Faculty of
Dentistry, Mahidol University, Bangkok, Thailand.
Erman Acar,
BioMediTech, Tampere University of Technology, Tampere, Finland.
Ulla Ruotsalainen,
BioMediTech, Tampere University of Technology, Tampere, Finland.
R.
Holland Cheng,
Department
of Molecular and Cellular Biology, University of California Davis, Davis,
California, United States of America,
Please see the link here: https://stm.bookpi.org/CTCB-V6/article/view/8770
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