The purpose of this work is to develop and validate a traditional reverse phase column-based, ion-pairing HPLC-UV technique for the detection of metformin in human plasma.
Between November 2014 and February 2015, the Department of Biomedical Sciences at the University of Medicine's "Mother Tereza" hospital centre undertook this experimental investigation.
The method used in this investigation to
identify metformin was ion-pair separation followed by UV detection on plasma
samples that had been deproteinized and dichloromethane washed. An analytical
LiChrocart® 100 RP 18 (125 x 4.0mm i.d., 5 m particle size) C18 column was used
to perform the separation. At an isocratic flow rate of 1.25 mL/minute, sodium
dodecyl sulphate (0.3%) and 10 mM sodium phosphate buffer (pH=6.0; 32.5:67.5,
v/v) were pumped as the mobile phase. Quantification was carried out at 236 nm
using a UV/Vis DAD. For metformin in plasma concentration ranges of 50–1600
ng/mL, the calibration curves were linear (r > 0.9998). With a minimal detection
limit of 18 ng/ml, the test enables the measurement of metformin for
therapeutic drug monitoring. The inter-day and intra-day assay coefficients of
variation fell within clinically significant limits. For all three of the
plasma quality control concentrations that were looked at, absolute recovery
was reported to be better than 90%. The suggested method for measuring
metformin in human plasma was discovered to be quick, exact, and accurate. This
method was successfully applied to an oral dosage pharmacokinetic study in
humans.
Author(s) Details:
Eva Troja,
Profarma SH.A. Pharmaceutical Industry, Tirana, Albania.
Leonard
Deda,
Department
of Biomedical Sciences, Faculty of Medicine, University of Medicine, Tirana,
Albania.
Gëzim Boçari,
Department of Biomedical Sciences, Faculty of Medicine, University
of Medicine, Tirana, Albania.
Please see the link here: https://stm.bookpi.org/CAPR-V7/article/view/8364
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