The aim of this study was to investigate the role of extracellular signal-regulated kinases (ERK1/2) and/or p53 activation in the apoptotic process induced by cisplatin-CisPT treatment on two head and neck squamous cell carcinoma cell lines (FaDu and PE/CA-PJ49) and how curcumin (CRM) used as an adjuvant supports it. According to data, CRM enhances CisPt activity. In both cell lines, CRM raised the phosphorylation of the p53 protein. CisPt altered the phosphorylation of the p53 protein in PE/CA-PJ49 cells but enhanced it in FaDu cells. The constitutive expression of activated ERK1/2 protein-kinase varied in the two tumour cell lines under investigation. The activation status of ERK1/2 was a factor in the cell processes that CisPt and/or CRM induced, including cell proliferation and death. Our results suggest that the phosphorylation of p53 during the apoptotic response to CRM treatment may need ERK1/2. In both tumour cell lines, combination treatments (CisPt and CRM) resulted in apoptosis that was dependent on p53 phosphorylation and ERK1/2 activation. Finally, ERK1/2 may affect cell proliferation and/or death depending on the type of therapeutic drug, the properties of the cells, and the level of ERK1/2 activation.
Author (s) Details
Marinela Bostan
Stefan S. Nicolau Institute of Virology,
Center of Immunology, Bucharest, Romania and Victor Babes National
Institute of Pathology, Bucharest, Romania.
Georgiana Gabriela
Petrica-Matei
Department of Cytogenetics, Personal
Genetics - Medical Genetics Center, Bucharest, Romania.
Gabriela Ion
Stefan S. Nicolau Institute of Virology,
Center of Immunology, Bucharest, Romania.
Nicoleta Radu
University of Agronomic Sciences and
Veterinary Medicine of Bucharest, Biotechnology Dept.& National Institute
for Chemistry and Petrochemistry R&D of Bucharest, Romania.
Mirela Mihaila
Stefan S. Nicolau Institute of Virology,
Center of Immunology, Bucharest, Romania.
Razvan Hainarosie
Prof. Dr. Dorin Hociota Institute of
Phonoaudiology and Functional ENT Surgery, Bucharest, Romania.
Lorelei Irina Brasoveanu
Stefan S. Nicolau Institute of Virology,
Center of Immunology, Bucharest, Romania.
Viviana Roman
Stefan S. Nicolau Institute of Virology,
Center of Immunology, Bucharest, Romania.
Carolina Constantin
Victor Babes National Institute of
Pathology, Bucharest, Romania.
Monica Teodora Neagu
Victor Babes National Institute of
Pathology, Bucharest, Romania.
Please see the link here:- https://stm.bookpi.org/CPMS-V8/article/view/7803
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