The goal of the current study was to identify the most responsive explants, determine the best doses of plant growth regulators, and identify additional physical characteristics that would accelerate in vitro morphogenesis by cultivating explants from nodal sections and leaf discs. Explants from the leaf disc and nodal section of Plumbago zeylanica were inoculated on basal media that had been mixed with various quantities and combinations of auxins and cytokinins, both individually and collectively. For the inoculated nodal segment, the culture medium MS2B (MS+2.0 mg l-1 BA) demonstrated better shoot proliferating competence (84.14%), but the nutrient medium MS2N.5B (MS+2. 0 mg l-1 NAA+ 0. 5 mg l-1 BA) demonstrated appropriate for higher degree of callus initiation (91.69%). While the nutritive medium MS2B.5N (MS+2.0 mg l-1 BA+0.5 mg l-1 NAA) produced more shoots (11.12) that were longer (7.11 cm). On culture medium MSN.5Td (MS+1.0mgl-1NAA + 0.5 mgl-1 TDZ), however, larger morphogenic calli formation (45.58%) and plantlets regeneration were achieved. For cultured leaf disc, inoculation media MS2N (MS+ 2.0 mgl-1NAA) produced callus in a higher percentage (91.12%). In the current study, it was found that MS medium supplemented with either IBA or NAA at a concentration of 0.1 mg l-1 was best for promoting a higher in vitro rooting response, including root proliferation, number(s), and length. In the field, regenerants were successfully established following hardening with typical phenotypic characteristics.
Author(s) Details:
M. K. Tripathi,
Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
G. Tiwari,
Department of Medicinal & Aromatic Plants, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India and Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Sushma Tiwari,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Niraj Tripathi,
Directorate of Research Services, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Mohini Sharma,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
Shashank Bhargav,
Department of Plant Molecular Biology and Biotechnology, College of Agriculture, Rajmata Vijayraje Scindia Agricultural University, Gwalior 474002, India.
S. L. Patidar,
Department of Medicinal & Aromatic Plants, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India.
Sharad Tiwari,
Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur 482004, India.
Please see the link here: https://stm.bookpi.org/RABS-V7/article/view/8046
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