Pathogens that are resistant to conventional antibiotics are becoming a bigger hazard and are getting better at avoiding them. Finding new antimicrobials is therefore essential to tackling the issues we are currently facing. The most effective producer of antibiotics among the other producers was Bacillus subtilis subsp. subtilis 168, which was isolated from a soil sample collected from a river bank in Abuja, Nigeria. The current study's goals were to (i) use the broth microdilution method to test the extract and fractions of the candidate isolate for antibacterial activity, and (ii) use gas chromatography-mass spectrometry (GC-MS) to identify the bioactive chemicals. We determined the MBC/MIC ratio, the minimum bactericidal concentration (MBC), and the minimum inhibitory concentration (MIC). The ethyl-acetate extract was effective against all pathogens tested (MIC range: 28.70–57.40 mg/ml). The extract showed bactericidal effectiveness against all tested pathogens (MBC/MIC between 1.00 and 2.00); Proteus mirabilis was the least susceptible pathogen. The extract was separated using vacuum liquid chromatography, and after being purified, pathogenic strains were exposed to the fractions. Against all of the test bacteria (MIC between 0.09 mg/ml and 0.75 mg/ml), fraction E was the most efficacious fraction and also the most bactericidal (MBC/MIC between 2.00 and 2.11). The pure subfraction obtained from fraction E was analyzed using GC-MS, which revealed 13 chemicals with varied retention periods and peak areas. Three of these were particularly significant: (i) bis(2-ethylhexyl) phthalate; (ii) 1,4-epoxynaphthalene-1(2H)-methanol; and (iii) 4,5,7-tris. -3,4-dihydro- Neogammacer-5-en-3-ol, 3.beta, D:B-Friedo. The locally obtained Bacillus subtilis subsp. subtilis 168 may be a rich source of lead compounds for use in biotechnology and medicine, according to our research.
Author(s) Details:
Olasinbo Olumuyiwa Balogun,
Department of Clinical Laboratory Services, Institute of Human Virology Nigeria, IHV-N Campus. FCT, Abuja, Nigeria.
Sylvanus Chukwudi Ugoh,
Department of Microbiology, University of Abuja, Abuja, Nigeria.
Peters Oluwale Oladosu,
Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development (NIPRD), Abuja, Nigeria.
Please see the link here: https://stm.bookpi.org/IMB-V7/article/view/8074
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