The goal of the current study was to investigate the relationships between various types of allergies and the genes immunoglobulin-E (IgE), interleukin-4 (IL-4), and interleukin-13 (IL-13), as well as their various variants. An expert team used a pre-tested questionnaire to compile data on diet, way of life, and clinical conditions. A 2ml blood sample from each was taken using coagulation and anticoagulation vials. Serum and DNA samples were taken and saved for further use. In contrast to ELISA, which was utilised to measure serum IgE, PCR-RFLP techniques were used for the genotypic investigation. Between patients and controls, there was a statistically significant difference in the serum IgE levels (P 0.05). Subjects with different genotypes of the IL-4 and IL-13 genes retained the observed difference in serum IgE levels (P 0.05). Furthermore, the aforementioned genetic variations significantly changed the risk of allergy when allergy risk was stratified based on different clinical traits. In conclusion, our results show that elevated IgE levels are substantially related with a range of allergies in the study group, along with variant forms of the IL-4 and IL-13 genes.
Author(s) Details:
Taha Ashraf Qurashi,
Department of Clinical Biochemistry, SKIMS, Srinagar-190011, India.
Gulzar Ahmad Bhat,
Department of Clinical Biochemistry, SKIMS, Srinagar-190011, India.
Syed Mudassar,
Department of Clinical Biochemistry, SKIMS, Srinagar-190011, India.
Please see the link here: https://stm.bookpi.org/RABS-V7/article/view/8044
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