For simultaneous quantification of Hydroquinone, Hydrocortisone, and Tretenoin from their Cream Formulation, a simple, sensitive, quick, exact, and accurate stability indicating RP-HPLC method has been devised.
Methods: The chromatographic separation was carried out on a reversed-phase Inertsil C18 (4.6 mm I.D. 250 mm, 5 m)column with a mobile phase of Buffer (pH 4.0) 0.05M potassium dihydrogen ortho phosphate-Methanol in an 80:20 percent V/V ratio at a flow rate of 1 ml/min and UV detection at max 265 nm. The approach demonstrated linearity, with correlation coefficients of 0.998, 0.998, and 0.996 for Hydroquinone, Hydrocortisone, and Tretenoin, respectively, over the ranges of 40-120 g/ml, 20-60 g/ml, and 0.25-0.75 g/ml.
The retention times for Hydroquinone, Hydrocortisone, and Tretenoin were 3 minutes, 5 minutes, and 6 minutes, respectively. For all of the components, the mean recoveries were determined to be in the range of 97.00–101.00 percent. The approach was validated in accordance with the International Conference on Harmonization (ICH) requirements. The approach was validated in accordance with the International Conference on Harmonization (ICH) requirements.
Conclusion: It can be concluded that using the approach can save a significant amount of time and money, and that it can be utilised in small laboratories with high precision and a broad linear range. The procedure was stable and specific, and no degradants interfered when the sample was stressed under various circumstances such as acid, base, oxidative, thermal, and photolytic.Author(S) Details
Raksha Shilu
School of Pharmacy, RK University, Rajkot, India. and Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India.
Pankaj Kapupara
School of Pharmacy, RK University, Rajkot, India.
Meghna Patel
Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India.
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