Study on Cloning, Sequencing and Phylogenetic Analysis of Granulocyte Macrophage Colony Stimulating Factor of Indian Cattle (Bos indicus) | chapter 3 | Research Aspects in Agriculture and Veterinary Science Vol. 2

 The cDNA coding for Granulocyte macrophage colony stimulating factor (GM-CSF) from LPS activated macrophages was amplified using reverse transcriptase polymerase chain reaction (RT-PCR), which was then cloned into the pCNDA vector and sequenced. The amplified bovine GM-CSF cDNA was 755 bp length without the secretory signal peptide sequence. The ORF coded for 143 amino acids with a molecular weight of 16200 Da and was 99 percent identical to Bos taurus and Bubalus bubalis, respectively. The phylogenetic study found that the GM-CSF sequences of swine and horse are the most distinct from those of buffalo, sheep, goat, and deer.

Author (S) Details

Ramya Kalaivanan

Veterinary College and Research Institute, Namakkal-02, India. and Indian Veterinary Research Institute, Hebbal, India.

P. Sankar

Veterinary College and Research Institute, Namakkal-02, India.

Subodh Kishore

Indian Veterinary Research Institute, Hebbal, India.

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