A simple, sensitive, quick, and cost-effective thin layer chromatographic technique was used to determine the concentrations of telmisartan and hydrochlorothiazide in human plasma using paracetamol as an internal reference. A combination of hydrochlorothiazide (HCT) and telmisartan (TEL) is more successful than either medicine alone in the treatment of hypertension.
The plasma sample was collected using a methanol-acetonitrile (3.0:0.1, v/v) mixture. Concentrations of 200, 400, 600, 800, 1000, and 1200 ng/spot were employed for the hydrochlorothiazide and telmisartan calibration curves, respectively. Telmisartan and hydrochlorothiazide, respectively, showed 75.98 percent and 81.91 percent recovery. Chloroform: methanol: toluene (8:2:4 v/v/v) makes up the mobile phase. At a wavelength of 278 nm, densitometric analysis was performed. The Rf values for hydrochlorothiazide, paracetamol, and telmisartan, respectively, were 0.28 0.05, 0.50 0.05, and 0.66 0.05. The stability of telmisartan and hydrochlorothiazide in plasma was validated in three freeze-thaw cycles (20 C), on bench for 24 hours, and post preparative for 48 hours. A recovery study was used to statistically validate the recommended method for identifying telmisartan and hydrochlorothiazide in human plasma. The method is less expensive and faster than previously disclosed approaches. In the future, we may be able to use this technique for bioequivalence research.Author (s) Details
Ambadas R. Rote
Department of Pharmaceutical Chemistry, MGV's Pharmacy College, Pune University, Nashik, India.
Poonam R. Sonavane
Department of Pharmaceutical Chemistry, MGV's Pharmacy College, Pune University, Nashik, India.
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