Glucose
oxidase is an intracellular and extracellular enzyme of the genus Aspergillus
and Penicillium, developed on an industrial scale. The purpose of this research
was to study the isolation of Aspergillus niger glucose oxidase (GOx) in an
aqueous two-phase system consisting of PEG 7500 (150 g l-1), potassium
phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), divided into
polymer phase enzymes. GOx (69.2 percent) was recovered by sequential
extraction by 11.8 fold purification in the polymer process, giving a yield of
129U mg protein-1.
Author(s) Details
Dr. Jagdish Singh
Department
of Biotechnology, Mata Gujri College Sri Fatehgarh Sahib - 140406 Punjab,
India.
Ms. Surbhi Goyal
View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/353
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