Objectives: Knowledge of genetic parameters of traits, such as heritabilities and genetic correlations, is important for an effective potato breeding strategy, hence the need to test genetic variability estimates of yield-related traits in Irish potato mutants Study Design: There was no replication of mutant minitubers at M1V1 generation because each does not maintain the same genetic minituber. The tubers were repeated in alpha lattice design 3 times in the generation of M1V2 and M1V3. Location and Period of Study: Irradiation was conducted at IAEA / FAO Seibersdorf Plant Genetics and Breeding Laboratories (PGBL), Vienna, Austria. The mutant microtubers (consisting of Asante, Mpya and Sherekea) were transported to Eldoret University, Kenya, for establishment between April 2015 and March 2017 following mutation induction. Methodology: A total of 30 tubers were dispatched to irradiate each of the three potatoes. Irradiation of in vitro nodal cuttings (without leaf) followed by in vitro shoot propagation and irradiation of in vitro nodal cuttings (with leaf) followed by direct in vitro micro-tuber development have been produced in two in-vitro radio-sensitivity tests involving different tissues. A total of 570 mutant microtubers (Asante 230, Mpya 160, Sherekea 180) were produced from the three potato varieties after mutation induction and were transported to Kenya to be formed at the University of Eldoret. In the greenhouse, the M1V1 microtubers were created, while the M1V2 and M1V3 mutant generations were planted in the research field at the University of Eldoret. Results: It revealed that in Mpya and Sherekea mutants, the highest positive heritability percentage (H2) estimates were 81.51 percent and 87.7 percent respectively in plant height. Conclusion: High heritability estimates of tuber numbers indicate that induced mutation has been effective in the production of new potato genotypes that will be used in future breeding programmes. The present study indicates that different genetic variability estimates were shown by the potato mutants. It was found that the number of tubers within the mutant populations exhibited high estimates of heritability. The induced mutation succeeded in producing new genotypes of potatoes that could be used in potential breeding programmes.
Author(s) Details
Dr. Emmy Chepkoech
School of Agriculture and Biotechnology, University of Eldoret, P.O.Box
1125-30100, Eldoret, Kenya.
School of Agriculture and Biotechnology, University of Eldoret, P.O.Box 1125-30100, Eldoret, Kenya.
Miriam G. Kinyua
School of Agriculture and Biotechnology, University of Eldoret, P.O.Box 1125-30100, Eldoret, Kenya.
Oliver Kiplagat
School of Agriculture and Biotechnology, University of Eldoret, P.O.Box 1125-30100, Eldoret, Kenya.
Julius Ochuodho
School of Agriculture and Biotechnology, University of Eldoret, P.O.Box 1125-30100, Eldoret, Kenya.
Leah Boit
School of Agriculture and Biotechnology, University of Eldoret, P.O.Box 1125-30100, Eldoret, Kenya.
View Book :- https://bp.bookpi.org/index.php/bpi/catalog/book/306
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