Cellulose is the largest waste component, thus creating a
serious pollution problem. Anaerobic bacteria producing cellulases have not
been extensively studied though they are the efficient producers of cellulase.
The anaerobic cellulolytic bacterium, Clostridium papyrosolvens was isolated
from a consortium developed in the laboratory from the compost sample collected
in the surroundings of Mysore, Karnataka, India. Clostridium papyrosolvens
CFR-1010, an anaerobic cellulolytic bacterial strain was identified as a potent
cellulase producer and selected for the purification and kinetic studies of
Exo-𝛽-1,4-glucanase
and Endo-𝛽-1,4- glucanase. The molecular masses of the
enzymes were 65 and 60 kDa, respectively. The enzymes showed maximum activities
at pH 5.0 and at a temperature of 50oC. The activities increased in the
presence of MnCl2, whereas, N-bromosuccinimide decreased enzyme activities by
68 and 75% respectively, thus suggesting the presence of tryptophan residues at
the active sites of enzymes. Exoglucanase had a Km of 20 mg/ml and Vmax of 22
units/min/mg of protein whereas the endoglucanase exhibited a Km of 6.66mg/ml
and Vmax of 11.76 units/min/mg of protein. Results of the present studies
suggest the use of C. papyrosolvens for cellulase production in shorter periods
of time and they also add significance for the exploration of this organism for
industrial applications. The exo 𝛽-1,4-glucanse and endo-𝛽-1,4-glucanase
from C. papyrosolvens CFR-1010 are applicable for the biodegradation of
cellulosic wastes which can be used in the biogas digesters and for the
commercial production of glucose or various other fermentation products.
Author(s) Details
Sharmila Tirumale
Department of Food Microbiology, Central Food Technological
Research Institute, Mysore - 570 020, India.
K. Soumya
Department of Microbiology, Jnanabharati Campus, Bangalore
University, Bangalore - 560 056, India.
G. L. Sreelatha
Department of Microbiology, Jnanabharati Campus, Bangalore
University, Bangalore - 560 056, India.
Krishnanand
Food Microbiology, CFTRI, Mysore, 570020, India.
Please see the link:- https://doi.org/10.9734/bpi/rpmab/v4/182
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