Organ and tissue Transplantation have been introduced from
time immemorial. Renal Transplantation is the most common among the solid organ
transplantations. Renal transplantation is the primary treatment modality for
end-stage renal disease. People are currently suffering from a global organ
crisis, which further makes the renal transplantation process even more crucial
and time-consuming. This study evaluates the HLA typing (HLA-A,-B, DR) in
chronic kidney disease patients who attended a tertiary care hospital in the
period from 2019 to 2023 for pre-renal transplantation workup. In this study, only the living Donor
transplantation was considered. In 7 patients, only the disease was detected
early. The transplantation was done before any need for dialysis. Mostly they
were related donor. 45 cases were unrelated donors. In this study, the PCR SSP
method was used. Histocompatibility testing is the most basic part of renal
transplantation. Tissue typing and cross-matching are the main tests done
before transplant. The recipient donor compatibility can be assessed by this
method for successful transplantation.
DNA-based HLA typing is now popular everywhere. Serological HLA typing,
which was used previously, has now become obsolete and replaced by DNA
typing. Complement Dependent
Cytotoxicity ( CDC) Crossmatch plays an important role in every transplant.
Flow Cytometry Crossmatch is also used in some centres. Virtual cross-match is
also one type of sophisticated cross-match where the recipient’s antibody
profile and the Donor’s HLA type are assessed virtually using a solid-phase
assay. So HLA typing and cross-matching are the most important investigations
for graft survival and to avoid graft rejection. Assessment of PRA and DSA also
gives useful information regarding management.
Author(s) Details
Madhumita
Mukhopadhyay
Department of Pathology, IPGME&R, Kolkata, India and JISMSR, Howrah,
West Bengal, India.
Shivangi Kailash
Department of Pathology, IPGME&R, Kolkata, India.
Please see the book here:- https://doi.org/10.9734/bpi/msraa/v9/5983
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