Mango products sold in Yola and the neighborhood exhibit symptoms of the anthracnose disease. Therefore, the study focused on seclusion, characterization, identification and pathogenicity experiment of fungal pathogen guide the disease in Yola, Adamawa State, Northeastern Nigeria. Mango crops and leaves that were naturally infected accompanying anthracnose were purposefully sampled from home flowers, farms, and marketplaces. The naturally infected plant parts appearance the disease syndromes were taken and proposed to the laboratory for direct isolation, description, identification of the fresh pathogen and pathogenicity testing of fungal isolates. 5 mm2 pieces of indicative crown tissue were inspired from the advancing injury margins, sterilized accompanying 70% ethanol for 2 brief time period, then with 10% sodium hypochlorite for 1 brief time period and lastly rinsed in 3 changes of clean distilled water. The completely clean lesion cut piece were placed on each petri bowl containing the hardened PDA. A plug of sporulating mycelia was taken each plate, mixed with 2 drops of Lactophenol cotton sad “RM” stain, (Biolution Resources), covered accompanying a slide cover, was used for deciding spore shape and number of septa. Colonies bearing same countenance were grouped together and identified as alike species. Nineteen fungal communities from three genera were obtained from the sampled instinctively infected unhealthy plant parts. They are; Aspergillus niger, Rhizopus oryzae, and Colletotrichum gloeosporioides were recognized located the morphological similarities; color, quality, spore shape and magnitude, and number of septa. The frequency of C. gloeosporioides was highest (66.67%) (77.78%) in two together infected products and leaves, followed by A. niger (11.11% and 20.00%) and R. oryzae (22.22% and 10%), individually. Of the three isolates, only one (C. gloeosporioides), was found expected pathogenic, by its skill to incite anthracnose disease on the specific healthy plant part.
Author(s) Details:
Aishatu Haruna,
Department of Crop Protection, Modibbo Adama
University Yola, Adamawa State, Nigeria.
Fadimatu
A. Jika,
Department
of Crop Protection, Modibbo Adama University Yola, Adamawa State, Nigeria.
Mahmud Y. Jada,
Department of Crop Protection, Modibbo Adama University Yola, Adamawa
State, Nigeria.
Gali A. Ishaku,
Department of Biotechnology, Modibbo Adama University Yola, Adamawa State,
Nigeria.
Please see the link here: https://stm.bookpi.org/EIAS-V4/article/view/10813
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