Investigation of Aspartate Aminotransferase and Alanine Aminotransferase Detection on Paper-Based Analytical Devices with Inkjet Printer-Sprayed Reagents | Chapter 4 | New Innovations in Chemistry and Biochemistry Vol.7

 Pipette titration is used to detect general biochemistry on paper-based microanalytical devices (PADs). However, for large-scale detection operations, such a technique is exceedingly time demanding. Furthermore, while automated methods for boosting the efficiency of large-scale PAD manufacture are available, the associated equipment is quite costly. As a result, this research suggests a low-cost technique for PAD production that involves using a modified inkjet printer to apply the reagent. Given AST and ALT concentrations in the range of 5.4 to 91.2 U/L (R2 = 0.9932) and 5.38 to 86.1 U/L (R2 = 0.9944), the best reaction times for detection of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are demonstrated to be 6 and 7 minutes, respectively. The experimental findings obtained utilising the proposed PADs for the concentration detection of AST and ALT in real human blood serum samples were found to be in good agreement with those obtained by National Cheng Kung University hospital using a traditional spectrophotometric detection approach.

Author(s) Details:

Hsiang-Li Wang,
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.

Chien-Hung Chu,
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.

Sing-Jyun Tsai,
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.

Ruey-Jen Yang,
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.

Please see the link here: https://stm.bookpi.org/NICB-V7/article/view/5920