Loop-Mediated Isothermal Amplification-Immunochromatography (LAMP-IC) Assay for Rapid Halal Food Authentication | Chapter 5 | Food Science and Agriculture: Research Highlights Vol. 2

Rising consumer demand for healthy, contaminant-free food, along with increased interest in halal certification, has underscored the need for reliable food authentication methods. Loop-mediated isothermal amplification (LAMP) is a rapid and efficient DNA amplification method that has been widely applied in various fields, including halal food authentication. This chapter highlights the development of immunochromatographic (IC) strip tests and LAMP-based nucleotide detection combined with IC (LAMP-IC) as in-house halal authentication kits. IC strip tests were designed to detect pork adulteration in beef or chicken mixtures, achieving sensitivity up to 1/5,000–1/10,000 in raw samples, though they are currently ineffective for cooked foods. In contrast, LAMP-IC effectively detects dog and pig DNA in cooked meat (boiled at 100°C for 15 minutes) within a single reaction at 68°C for 30 minutes, followed by IC strip analysis in 5–30 minutes. These findings demonstrate LAMP-IC as a practical and promising tool for halal food authentication.

 

Author (s) Details

Sulaiman Ngongu Depamede
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62 Mataram 83625, Indonesia.

 

Made Sriasih
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62 Mataram 83625, Indonesia.

 

Djoko Kisworo
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62 Mataram 83625, Indonesia.

 

Please see the book here:- https://doi.org/10.9734/bpi/fsarh/v2/5431

Serological Diagnosis of Dengue Fever and Its Correlation with Platelet Counts In a Tertiary Hospital, Nellore, Andhra Pradesh, South India | Chapter 4 | Highlights on Medicine and Medical Research Vol. 15

 Dengue fever is an Arboviral infection spread by mosquitos that primarily affects tropical and subtropical countries worldwide. Due to a lack of immune prophylaxis and specific antiviral therapy, outbreaks occur almost every year. To avoid morbidity and mortality, a prompt diagnosis is required. Classical Dengue fever symptoms include a high fever, severe headache, chills, and generalized muscle and joint pain. The purpose of this study was to determine the dengue virus seroprevalence in a tertiary care hospital in Nellore. Serological tests were carried out on blood samples submitted to the microbiology laboratory. Between June and October of 2015, suspected dengue infections were sent to a lab. A solid phase immunochromatographic technique was used to detect NS1 antigen, IgG, and IgM antibodies in a Dengue Day 1 Test kit (J. Mitra & Co. Pvt Ltd). NS1 antigen positivity was found in 88.69% of the samples, 3.41 % for IgM, and 2.77 % for IgG. Thrombocytopenia was discovered in 71.42 percent of patients, with the highest rate found in those who were only positive for the NS1 antigen (301 cases). Because it combines antigen and antibody assays on a single serum sample and is simple and quick to perform, immunochromatography is the preferred test for making an accurate diagnosis.

 
Author (S) Details

M. Shabnum
Department of Microbiology, Narayana Medical College, Nellore - 524002, Andhra Pradesh, India.

P. Sreenivasulu Reddy
Department of Microbiology, Narayana Medical College, Nellore - 524002, Andhra Pradesh, India.

P. Vasundhara Devi
Department of Microbiology, Narayana Medical College, Nellore - 524002, Andhra Pradesh, India.

View Book :- https://stm.bookpi.org/HMMR-V15/article/view/1954