Rising consumer demand for healthy, contaminant-free food, along with increased interest in halal certification, has underscored the need for reliable food authentication methods. Loop-mediated isothermal amplification (LAMP) is a rapid and efficient DNA amplification method that has been widely applied in various fields, including halal food authentication. This chapter highlights the development of immunochromatographic (IC) strip tests and LAMP-based nucleotide detection combined with IC (LAMP-IC) as in-house halal authentication kits. IC strip tests were designed to detect pork adulteration in beef or chicken mixtures, achieving sensitivity up to 1/5,000–1/10,000 in raw samples, though they are currently ineffective for cooked foods. In contrast, LAMP-IC effectively detects dog and pig DNA in cooked meat (boiled at 100°C for 15 minutes) within a single reaction at 68°C for 30 minutes, followed by IC strip analysis in 5–30 minutes. These findings demonstrate LAMP-IC as a practical and promising tool for halal food authentication.
Author
(s) Details
Sulaiman Ngongu
Depamede
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62
Mataram 83625, Indonesia.
Made Sriasih
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62
Mataram 83625, Indonesia.
Djoko Kisworo
Faculty of Animal Science, University of Mataram, Jl. Majapahit No. 62
Mataram 83625, Indonesia.
Please see the book here:- https://doi.org/10.9734/bpi/fsarh/v2/5431
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