Background: Burns are dynamic wounds that may present a
progressive deepening and expansion of the initial burn area, a process defined
as burn wound progression. There are multiple factors involved in the
pathophysiology of burn wound progression, however, the exact mechanisms are
unclear and the therapeutic modalities are limited. This study is aimed to
investigate the effect of epigallocatechin gallate on experimental burn wound
progression.
Methods: A deep second-degree burn was produced on male Wistar
rats. Epigallocatechin gallate was systemically administrated as a treatment
intervention. Mitochondrial DNA level in serum and level of proinflammatory
cytokines in burn wounds was detected. Malonaldehyde content, myeloperoxidase
activity, and the nucleotide-binding oligomerization domain-like receptor
family, pyrin domain containing 3 inflammasome levels in the burn wounds were
measured. The histopathological examination of burn wounds was performed and
the time to wound re-epithelialization was recorded.
Results: Burn resulted in remarkably higher levels of
mitochondrial DNA release in serum and proinflammatory cytokines in burn
wounds. Moreover, the malonaldehyde content, myeloperoxidase activity, and
nucleotide-binding oligomerization domain-like receptor family, pyrin domain
containing 3 inflammasome level in burn wounds were significantly higher than
that of sham burn. Epigallocatechin gallate treatment significantly reduced
mitochondrial DNA levels in serum and inflammatory response in burn wounds.
Furthermore, the burn wound depth of rats in the epigallocatechin gallate group
was markedly attenuated and the wound re-epithelialization time was
accelerated.
Conclusion: Epigallocatechin gallate ameliorated burn wound
progression probably by inhibiting mitochondrial DNA-induced inflammation and
protecting wounds from inflammatory infiltration and oxidative damage.
Author
(s) Details
Mengjing Xiao
Department of Burn Plastic and Cosmetic Surgery, South China Hospital,
Medical School, Shenzhen University, Shenzhen, 518116, P.R. China.
Hongming Yang
Department of Burn Plastic and Cosmetic Surgery, South China Hospital,
Medical School, Shenzhen University, Shenzhen, 518116, P.R. China.
Ruichen Wang
Department of Burn Plastic and Cosmetic Surgery, South China Hospital,
Medical School, Shenzhen University, Shenzhen, 518116, P.R. China.
Please see the book here:- https://doi.org/10.9734/bpi/mmrnp/v9/2472
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