Acinetobacter
baumannii is a major opportunistic nosocomial pathogen. Sodium chloride is
one of the elements that is necessary for the survival of some bacterial
species. A. baumannii does not show a
high salt tolerance, unlike other salt-tolerant bacteria. The purpose of this study
is to identify the effect of NaCl on A.
baumannii. In this study two isolates of A. baumannii have been used, the local isolate HHR1 and the global
isolate ATCC 17904. The isolates were routinely cultured on Nutrient agar and
MacConkey agar media under aerobic conditions at 37˚C for 24 hours. Firstly,
the influence of NaCl on the growth of two isolates of A. baumannii was monitored and the effect of two concentrations of
NaCl on Lipopolysaccharide components was determined by SDS-PAGE. SDS-PAGE was
done by using the Bio-Rad system according to, using Promega (USA) system. 12 %
resolving gel and 4 % stacking gel were prepared and set. The result showed
that HHR1 after 20 hr of incubation period reached 0.6 at OD600 in the presence
of salt, while the control was continued, whereas, A. baumannii ATCC didn’t exert any defect growth. Moreover, NaCl
has greatly affected the LPS components, where only O-antigen has appeared
under 1% NaCl in A. baumannii HHR1
and 3% NaCl in A. baumannii ATCC.
Author
(s) Details
Nagham Saadi Mohammed
Department of Biology, College of Science for Women, University of Baghdad,
Baghdad, Iraq.
Halah H. A-Haideri
Department of Biology, College of Science for Women, University of Baghdad,
Baghdad, Iraq and Department of Pharmacology and Toxicology, College of
Pharmacy, Mustansiriyah University, Iraq.
Please see the book here:- https://doi.org/10.9734/bpi/crpbs/v3/2316
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