Nucleic Acid-based Approaches for Nonsense Suppression and Their Applications: An Update |Chapter 5 | Microbiology and Biotechnology Research: An Overview Vol. 5

Genetic modification and manipulation are valuable tools for improving our understanding of biological mechanisms. For decades, biologists have taken advantage of the molecular and genetic tools. One of these genetic methods, suppressor analysis, has been essential in helping us comprehend several biological processes and pathways, as well as identifying previously unknown combinatorial connections between genes and gene products. High-throughput techniques have helped in the identification of suppressors on a broad scale as technology has advanced over the years. They are increasingly being used in human genetics to treat various diseases, as well as in plant biology to improve crops and induce disease resistance. In the preceding section of this review, we look at some of the core principles and mechanisms of nucleic acid-based suppressors, followed by a detailed discussion of methods, approaches and limitations. We next discuss their applicability in human genetics and plant biology in depth. The review discusses the benefits of various approaches in the treatment of CNS illnesses, which have proven difficult for a variety of reasons. In plant biology, the use of answering basic questions and crop enhancement and disease resistance is explored. To summarise, nonsense suppression opens up new avenues for investigating fundamental biology questions like transcription and translation. The ever-expanding list of illness treatments in human genetics, the breadth of applications in plant sciences, and the newer toolkits all demonstrate the advantages of the method.

 

 

Author(s) Details

Mahalakshmi B.R
Department of Zoology, Government Science College, Nrupathunga University, Nrupathunga Road, Bangalore, 560001, India.

 

Priya M.D
Department of Zoology, Government Science College, Nrupathunga University, Nrupathunga Road, Bangalore, 560001, India.

 

Latha K
Department of Biotechnology, Maharanis Science College for Women, Autonomous JLB Road, Mysore, Affiliated to University of Mysore, Karnataka, India.

 

Ranjini. P
Department of Biotechnology, Sir MV Government Science College Bhadravathi, Karnataka, India.

 

Shailasree Sekhar
Department of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research, Mysore, India.

 

Shreya K.R.
Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore, India.

 

Kiran Kumar H.B.
Post-doc NCBS, Affiliated to Nrupathunga University, India.

 

Please see the book here :- https://doi.org/10.9734/bpi/mbrao/v5/6447