Mercury-based Traditional Medicines: Chemical Composition and Mineral-herb Mixtures Differ from Toxic Mercury Compounds on Hepatic Cytochrome P450| Chapter 5 | Recent Progress in Science and Technology Vol. 1

 Zuotai  (β-HgS)-holding 70 Wei-Zhen-Zhu-Wan (70W, Qishiwei, Rannasangpei) is a Chinese ethnomedicine at Qinghai-Tibet region for cardiovascular and gastrointestinal ailments.  We have found that 70W shielded against CCl4 hepatotoxicity. Utilizing cytochrome P450 (CYP), CCl4 is metabolized to create sensitive metabolites. It is unclear either 70W has any affect CYPs, and any specific impact should be distinguished to harmful planets orbiting sun compounds for safety evaluation.  In this experiment, Mice were given dispassionate doses of 70W (0.15-1.5 g/kg, po), Zuotai (30 mg/kg, po), and compared to HgCl2 (33.6 mg/kg, po) and MeHg (3.1 mg/kg, po) for 7 days. Liver RNA and protein were unique for qPCR and Western-blot analysis.The verdicts of this study granted that 70W and Zuotai had no belongings on hepatic mRNA expression of Cyp1a2, Cyp2b10, Cyp3a11, Cyp4a10 and Cyp7a1, and matching nuclear receptors [aryl hydrocarbon receptor (AhR), essential androstane receptor (CAR), pregnane X receptor (PXR), peroxisome proliferator-activated receptor-α (PPARα); farnesoid X receptor (FXR)]. In comparison, HgCl2 and MeHg raised mRNA expression of Cyp1a2, Cyp2b10, Cyp4a10 and Cyp7a1 except that Cyp3a11, and corresponding basic receptors except for PXR. Western-blot rooted mRNA results, showing increases in CYP1A2, CYP2B1, CYP2E1, CYP4A and CYP7A1 by HgCl2 and MeHg only, in addition treatments had no belongings on CYP3A.In contrast to HgCl2 and MeHg, Zuotai and Zuotai-containing 70W had little affect the hepatic CYPs and associated basic receptors. Thus, HgS-herb combinations differ from poisonous Hg compounds on hepatic P450 and nuclear receptors.

Author(s) Details:

Yu Nie,
Key Laboratory for Basic Pharmacology of Ministry of Education and the Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, China and Zunyi Blood Center, Zunyi, 563000, China.

Shang-Fu Xu,
Key Laboratory for Basic Pharmacology of Ministry of Education and the Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, China.

Yan-Liu Lu,
Key Laboratory for Basic Pharmacology of Ministry of Education and the Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, China.

Xiu-Rong Zhao,
Key Laboratory for Basic Pharmacology of Ministry of Education and the Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, China.

Cen Li,
Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001,China.

Li-Xin Wei,
Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001,China.

Jie Liu,
Key Laboratory for Basic Pharmacology of Ministry of Education and the Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, China.

Please see the link here: https://stm.bookpi.org/RPST-V1/article/view/9091