Evaluating the Diagnostic Performance of PCR Targeting Insertion Sequence (IS6110) for the Detection of Extra-pulmonary Tuberculosis| Chapter 7 | Research Advances in Microbiology and Biotechnology Vol. 2

 The disease of extra-pulmonary tuberculosis debris a challenge to control tuberculosis in spite of remarkable progress has been fashioned in diagnostics all along the last decade. The polymerase chemical reaction (PCR) was used in the disease of definitive extra pulmonary infection patients and to assess the accomplishment of insertion series (IS) 6110-based PCR assay as distinguished to conventional liquid education by Microbial Growth Indicator Tube (MGIT) 960 system. Patients accompanying clinically suspected extra-pulmonary TB provided 792 dispassionate specimens. There were 22 disciplined fluids, 69 pleural fluids, 240 cerebrospinal fluids (CSF), 386 endometrial tissues, 47 lymph nodes, 22 suppuration samples, 1 synovial fluid, 1 fallopian tube, 2 intelligence abscess, and 2 ovarian sac samples among the collections. All of these dispassionate samples were cultured on MGIT 960 tubes holding Modified Middlebrooks 7H9 broth medium and Auramine O staining (FM) for acid-fast bacterium (AFB). The Mycobacterium TB insertion series IS6110's 123 bp fragment was the target of the PCR. In our reasoning of 792 samples, we found that they were 87.5% sensitive to endometrial samples, 92.31% alert cerebrospinal fluid, 66.66% sensitive to pleural fluid, and 60% awake lymph node samples. Calculations show that the PCR IS6110 has a linked sensitivity and particularity of 85.71% and 82.91%, respectively. It is decided that PCR using IS6110 primer improved more positivity in extra-pulmonary samples distinguished to the conventional civilization method for detecting M. infection.

Author(s) Details:

R. Venkateswari,
Department of Medical Biochemistry, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

B. Usharani,
Department of Biomedical Genetics, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

P. Suganthi,
Department of Medical Microbiology, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

M. Muthuraj,
State TB Training and Demonstration Centre, Intermediate Reference Laboratory, Government Hospital for Chest Diseases, Puducherry, India.

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