Evaluating the Diagnostic Performance of PCR Targeting Insertion Sequence (IS6110) for the Detection of Extra-pulmonary Tuberculosis| Chapter 7 | Research Advances in Microbiology and Biotechnology Vol. 2

 The disease of extra-pulmonary tuberculosis debris a challenge to control tuberculosis in spite of remarkable progress has been fashioned in diagnostics all along the last decade. The polymerase chemical reaction (PCR) was used in the disease of definitive extra pulmonary infection patients and to assess the accomplishment of insertion series (IS) 6110-based PCR assay as distinguished to conventional liquid education by Microbial Growth Indicator Tube (MGIT) 960 system. Patients accompanying clinically suspected extra-pulmonary TB provided 792 dispassionate specimens. There were 22 disciplined fluids, 69 pleural fluids, 240 cerebrospinal fluids (CSF), 386 endometrial tissues, 47 lymph nodes, 22 suppuration samples, 1 synovial fluid, 1 fallopian tube, 2 intelligence abscess, and 2 ovarian sac samples among the collections. All of these dispassionate samples were cultured on MGIT 960 tubes holding Modified Middlebrooks 7H9 broth medium and Auramine O staining (FM) for acid-fast bacterium (AFB). The Mycobacterium TB insertion series IS6110's 123 bp fragment was the target of the PCR. In our reasoning of 792 samples, we found that they were 87.5% sensitive to endometrial samples, 92.31% alert cerebrospinal fluid, 66.66% sensitive to pleural fluid, and 60% awake lymph node samples. Calculations show that the PCR IS6110 has a linked sensitivity and particularity of 85.71% and 82.91%, respectively. It is decided that PCR using IS6110 primer improved more positivity in extra-pulmonary samples distinguished to the conventional civilization method for detecting M. infection.

Author(s) Details:

R. Venkateswari,
Department of Medical Biochemistry, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

B. Usharani,
Department of Biomedical Genetics, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

P. Suganthi,
Department of Medical Microbiology, Institute of Basic Medical Sciences, University of Madras, Tamil Nadu, India.

M. Muthuraj,
State TB Training and Demonstration Centre, Intermediate Reference Laboratory, Government Hospital for Chest Diseases, Puducherry, India.

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Determination of Clinical Profile of Childhood Extrapulmonary Tuberculosis | Chapter 18 | Recent Developments in Medicine and Medical Research Vol. 8

 Background: Tuberculosis is the world's second most common cause of infectious disease death, trailing only AIDS. The prevalence of all kinds of tuberculosis among children in India is unknown; most surveys have concentrated on pulmonary tuberculosis. The goal of this study was to look at the clinical profile of different forms of childhood EPTB. The goal of this study was to look at the clinico-epidemiological profile of different types of childhood EPTB.

The goal of this study was to look at how different forms of extrapulmonary tuberculosis affect children as they become older, as well as to look at the demographic profile and diverse clinical presentations of paediatric extrapulmonary tuberculosis.

Methods: A retrospective review of the clinical profiles of 100 children with EPTB aged 6 months to 12 years.

Results: 62 percent of the cases in our study were aged 0-5 years, 38 percent were aged 5-12 years (P = 0.041), with a male to female ratio of 1.9:1. 96 percent (P = 0.016) of the patients were from a lower socioeconomic level (P = 0.01). TBME (46%) was the most common kind of EPTB, followed by disseminated TB (21%), pleural effusion (12%), abdominal TB (10%), TB lymphadenitis (7%), and osteoarticular TB (2%). (4 percent ). Mild to moderate malnutrition (PEM Grades I, II) affected 28% of the patients, while severe malnutrition affected 46% (PEM Grades III, IV). BCG vaccination was given to 66% of the patients, and there was a history of Koch's contact in 28% of the cases. In 97 percent of CNS tuberculosis patients, fever was present, followed by altered sensorium and convulsion in 80%, tonic posture in 60%, and aberrant movements in 4%. Tonic posturing was the most common indication in 60% of cases, followed by the crack pot sign in 41%. Fever (100%), anorexia (90%), weight loss (80%), abdominal discomfort (50%) and hepatomegaly were all common symptoms in 100 percent of cases of abdominal TB.

Conclusion: EPTB among children over the age of one year, children from lower socioeconomic strata, and children who are extremely malnourished are the most common. Fever, altered sensorium, convulsions, and aberrant movements characterise CNS TB, whereas fever, anorexia, weight loss, and stomach pain characterise abdominal tuberculosis.

Author(S) Details

Dhara K. Gosai
Department of Pediatrics, B. J. Medical College and Civil Hospital, Ahmedabad, Gujarat, India.

Jigar B. Gosai
Department of Emergency Medicine, B. J. Medical College and Civil Hospital, Ahmedabad, Gujarat, India.

Omprakash S. Shukla
Department of Pediatrics, Baroda Medical College and S. S. G. Hospital, Vadodara, Gujarat, India.

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