Does the DNA Protective Capacity of Lilium candidum Extract and its Components Depend on the Experimental Design?| Chapter 7 | Cutting Edge Research in Biology Vol. 3

 Here we have forwarded two main questions: Whether the bioactivity of Lilium candidum extract and its main elements - kaempferol and jatropham depend on the exploratory design? How do kaempferol and jatropham as constituents of Lilium candidum total extract enhance its bioactivity?These questions have outlined our main objectives to purify: 1) the contribution of exploratory design to the DNA protective potential of Lilium candidum extract and allure main constituents kaempferol and jatropham; 2) the offering of kaempferol and jatropham to the DNA protective potential of Lilium candidum total extract.Chlamydomonas reinhardtii container-wall-less strain CW15 from the Culture Collection of Algae and Protozoa (CCAP), Ambleside, UK, was secondhand as a model object. To evaluate either the experimental environments can affect the bioactivity of Lilium candidum total extract and kaempferol and jatropham three exploratory designs were performed: linked treatment; split situation without the bury-treatment occasion; split treatment accompanying 4-hour bury-treatment occasion. The kinetics of double-fiber breaks (DSBs) repair was calculated at the 2nd and 4th hour of the improvement time, and the importance of DSBs repair capacity was determined.New evidence was provided that Lilium candidum total extract and allure components – kaempferol and jatropham own DNA protective ability increasing the size of cells' repair ability. This effect was strongly contingent on the experimental design.Our results help the concept that the bioactivity of plant extracts maybe due to cooperative effects betwixt individual components. Our verdict that in some exploratory conditions (design 2), Lilium candidum extract acted not possess DNA securing potential against zeocin-induced DSBs, even though two of its main constituents had aforementioned capacity, maddened new questions. They still remain open.

Author(s) Details:

Stephka Chankova,
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.

Teodora Todorova,
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.

Petya Parvanova,
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.

Please see the link here: https://stm.bookpi.org/CERB-V3/article/view/9199