Pseuderanthemum bicolor usually called limang-sugat owned by the family Acanthaceae. Leaves of P.bicolor were extracted accompanying methanol, kill and ethyl acetate. The extracts were subjected for beginning-amylase inhibition assay and hooking up study using GC-MS analysis result. Leaf extracts were willing by decoction arrangement and have been judged for alpha-amylase restriction assay and protein-ligand docking study against alpha-amylase protein. Among the three various extracts most significant substance causing chemicals to split into simpler substances inhibition was accompanied by ethyl acetate extract with maximal inhibition of 43.1% at 1mg/ml concentration. The remark drug acarbose revealed 96% hindrance at 0.5mg/ml concentration. The big bioactive compounds obtained from intoxicating, chloroform and ethyl acetate extracts were 1,6;2,3-Dianhydro-4-Deoxy-Beta-D-Ribo-Hexopyranose, Pseduosarsasapogenin-5,20-Dien,Methyl Ether/ Hexatriacontane, Di-N-Decylsulfone/Octadecanal, Squalene respectively. A total of 19 subordinate metabolites were tested for protein-ligand berthing against alpha-amylase protein, accompanying the reference drug acarbose professed a binding energy of -7.8 Kcal/mol and forming 20 hydrogen bonds accompanying the enzyme. Among the 19 test ligands, ‘2,2-Dibromocholestanone’from ethyl acetate extract personified highest separation energy of -9.3 Kcal/mol. The next highest extraordinary inhibition was showed by ‘Pseduosarsasapogenin-5,20-Dien Methyl Ether’ present in the intoxicating extract, with a separation energy of -9.3 Kcal/mol with the establishment of 2 hydrogen bonds. From the result it could be decided that the P. bicolor contain effective bioactive compounds, needs further research on pharmacological facets to develop a novel drugs.
Author(s) Details:
B. S. Ramesh,
Department
of Botany, Nrupathunga University, Bengaluru, India.
Please see the link here: https://stm.bookpi.org/CERB-V2/article/view/8888
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