A new reversed-phase high performance liquid chromatography method was developed for the simultaneous detection of Fexofenadine hydrochloride (FEXO) and Montelukast sodium (MONTE) in combination tablet formulation. Using a C18 Grace Column (4.6 250 mm, 5 um) as stationary phase and Methanol and H2O (70:30, v/v) as mobile phase, effective separation was obtained using 0.05 percent Orthophosphoric acid with a PH of 2.5 and a flow rate of 1 ml/min. The UV 730 D PDA detector was used for detection at 241.0 nm. The retention times for fexofenadine and Montelukast are 6.0167 minutes and 8.500 minutes, respectively. Throughout, the FEXO and MONTE were determined to be linear. 60 g/mL to 300 g/mL and 5 g/mL to 25 g/mL are the respective ranges. The approach that was created was exact, accurate, cost-effective, rapid, and selective. In the case of pharmaceuticals, the method yields satisfactory results. With good peaks, low tailing, good retention time, and appropriate resolution time between peaks, FEXO and MONTE were separated using the aforementioned procedure. As a result, this method was determined to be suitable and completed.
Author(s) Details
Government college of Pharmacy, Amravati, Maharashtra, India.
S. S. Khadabadi
Government college of Pharmacy, Amravati, Maharashtra, India.
S. C. Atram
Government college of Pharmacy, Amravati, Maharashtra, India.
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