The goal of this study was to compare the total phenolic (TPC) and flavonoid content (TFC) of five lesser-known fruits, including Aegle marmelos, Spondias pinnata, Limonia acidissima, Averhoa carambola, and Crescentia cujete, to Phyllanthus emblica (Amla), which is well-known for its antioxidant properties. Fruits include natural antioxidants that help to minimise oxidative stress. Antioxidant activities such as the 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) free radical scavenging assay, Ferric reducing antioxidant potential (FRAP) assay, and total antioxidant capacity were associated with total phenolic and flavonoid content of samples (TAC). The total phenolic content of each fruit extract was determined using the Folin-Ciocalteu method with certain modifications, and total flavonoids were calculated using the Aluminum trichloride colorimetric method. The TAC, FRAP, and DPPH antioxidant assays were all spectrophotometrically measured. Total phenolics were measured in milligrammes per 100 grammes of Gallic acid equivalent (mg GAE/100 gm), and total flavonoids were measured in milligrammes per 100 grammes of Quercetin equivalent (mg QE/100 gm). Spondias pinnata had the most TPC with 142.16 mg GAE/100 gm, whereas Phyllanthus emblica had the highest TFC with 91.1 mgQE/100 gm. DPPH radical scavenging activity was represented as a percentage (%), FRAP values as mg/100g Ascorbic equivalent (AAE), and total antioxidant activity as mg/100g Ascorbic equivalent (AAE). Spondias pinnata had the best DPPH radical scavenging activity (93.75 percent), Phyllanthus emblica had the greatest FRAP values (72.6 mg AAE/100 gm), and Spondias pinnata had the best overall antioxidant capacity (50.1 mg AAE/100 gm). Conclusion: The antioxidant activity of Spondias pinnata, an underutilised fruit, was found to be comparable to that of Phyllanthus emblica.
Author (s) DetailsDr. Sumita Dasgupta
Biotechnology Department, Bhagwan Mahavir College of Science and Technology, New City Light Road, Surat, India.
Ms. Maitry Pandya
Biotechnology Department, Bhagwan Mahavir College of Science and Technology, New City Light Road, Surat, India.
Mrs. Nirali Patel
Biotechnology Department, Bhagwan Mahavir College of Science and Technology, New City Light Road, Surat, India.
View Book : https://stm.bookpi.org/TIPR-V5/article/view/1238
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