Background: The growth of nanoparticles has a great footprint in recent years. There is increasing optimism that nanotechnology, as applied to medicine, will bring significant advances in the diagnosis and treatment of disease.
Objective: The objective of the study was to conduct a Green
synthesis of silver nanoparticles from aqueous extract of Pterocarpus marsupium bark and wood and its characterization. As
well as an in vitro anti-diabetic study.
Methods: The study was conducted using instruments, Magnetic
stirrer with hot plate, Magnetic bead, centrifuge (REMI), Shimadzu UV-visible
spectrophotometer, IR spectrophotometer, Malvern Zeta size analyser, and
scanning electron microscopy. The Pterocarpus
marsupium bark and wood were collected from Palakkad, Kerala, India.
Synthesis of Pterocarpus marsupium
silver nanoparticles was done by drop-wise addition of 1 mM silver nitrate to Pterocarpus marsupium bark and wood
extract. Characterization of synthesized nanoparticles was carried out by
visual examination, UV-visible absorption spectroscopy, FTIR spectroscopy, Poly
dispersity index and Zeta value. In vitro anti-diabetic study for the
synthesised silver nanoparticles was carried out by α-amylase inhibition assay.
Results: The result suggested that Pterocarpus marsupium exhibits good α- amylase inhibition under in
vitro conditions The synthesis of silver nanoparticles using Pterocarpus marsupium extract was
eco-friendly and cost-effective. Aqueous extract of Pterocarpus marsupium bark and wood extract were mixed with the
aqueous solution of silver nitrate. The colour of the solution changes from
yellow to brown colour due to the surface Plasmon resonance which is the
primary confirmation for the formation of silver nanoparticles. The surface
Plasmon band in the silver nanoparticles was found to be 431 nm. The FTIR study
concluded that hydroxyl and carboxyl groups act as reducing and stabilizing
agents and the phenolic group functions as a capping agent. The average
particle size was found to be 148.5 nm. Its polydispersity index was 0.336 and
zeta potential was measured and found to be -28 mV with the peak area of 100%
intensity. This indicates that the formed nanoparticles were stable. The in
vitro anti-diabetic study confirms that Pterocarpus
marsupium has anti-diabetic activity. From the FTIR spectral analysis, it
is concluded that the hydroxyl and carboxyl groups present may act as reducing
and stabilizing agents and the phenolic group present may act as a capping
agent.
Conclusion: Pterocarpus
marsupium silver nanoparticles are found to be effective for anti-diabetic
activity. The study found without using any chemical reagent as a stabilizing
agent the silver nanoparticles are stable. Hence this cost-effective and
eco-friendly method is highly promising for the future.
Author
(s) Details
J
Bagyalakshmi
Sri Ramakrishna Institute of Paramedical Sciences, College of
Pharmacy, Coimbatore, India.
H
Haritha
Sri Ramakrishna Institute of Paramedical Sciences, College of
Pharmacy, Coimbatore, India.
Please see the book here:- https://doi.org/10.9734/bpi/psnid/v1/2456
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