C-Phycocyanin (C-PC) is a phycobiliprotein found in
blue-green algae, such as Spirulina platensis which is often used as a dietary
nutritional supplement and exhibits a variety of pharmacological properties.
Cyanobacteria and algae possess a wide range of colored compounds, including
chlorophyll, carotenoids and phycobiliproteins. In this regard, extraction,
partial purification, antioxidant, anticoagulation and prevention of DNA damage
activity of C-PC were investigated. In the present study, a simple and
efficient method to extract C-PC from Spirulina platensis dry powder is
reported. Spirulina platensis dry powder was obtained from NB Laboratories Pvt.
Ltd., Nagpur, India. The extractions were carried out using two different
methods: cold maceration and sonication method. The extraction using the cold
maceration method proved to be the most efficient method. The efficiency of
extraction methods was determined by calculating the concentration and purity
ratio of isolated C-PC. Obtained crud C-PC was purified by ammonium sulphate precipitation,
dialysis and gel filtration and presented a final extraction yield of 3.27±0.09
mg/ml with a purity ratio of 2.317±0.08. When it was evaluated as an
antioxidant in vitro, it was able for scavenging nitric oxide. SDS-PAGE results
show that the 40% ammonium sulphate precipitated sample and dialyzed sample
show two bands of C-PC i.e. α and β with 17 and 19kDa respectively along with
other proteins. C-PC showed significant anticoagulation and prevention of DNA
damage activity. C-PC is a significant contributor to DNA damage prevention by
scavenging of –OH.
Author(s) Details:
Suresh P. Kamble,
Department of Biochemistry, Moolji Jaitha College (Autonomous),
Jalgaon, Maharashtra, India.
Please see the link here: https://stm.bookpi.org/ACPR-V6/article/view/13412
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