Background: An investigation was carried out on twelve clinical
cases of neoplasm of horn in bovines of Durg, Dhamtari and Rajnandgaon
districts of Chhattisgarh suspected of bovine horn core carcinoma (squamous
cell carcinoma) revealed the cytology, pathomorphology and immunohistochemical
(IHC) expression of Pan-cytokeratin (Pan-CK), p53 gene, epidermal growth factor
receptor (EGFR) and p16 gene in tumourous growth at horn in bovines.
Results: Eight out of 12 cases (66.66%) were confirmed as
SCC of horn on the basis of histopathological and immunohistochemical analysis.
Cytological examination of tumours by Papanicolaou staining revealed variation
in shape and size of cells and altered nuclear details. Grossly neoplasms of
horn revealed unilateral large cauliflower like growths at the base. SCCs were
classified as well, moderately and poorly differentiated types on the basis of
histopathology and immunohistochemistry (IHC). Well differentiated SCCs (n=4;
50%) were characterized by severe keratinization of horn epithelium with
concentric arrangement forming keratin pearls also called as “cell nests”.
Tumour islands of irregular shape observed in the horn epithelium invaded deep
into dermis layer. Moderately differentiated SCCs (n=2; 25%) characterized by
small keratin pearl formations and mitotic figures. Poorly differentiated SCCs
of horn (n=2; 25%) revealed absence of distinctive keratin pearls although deep
invasion from primary site was observed. SCC of horn revealed strong
immunohistochemical staining of Pan-CK, p53 and EGFR and negative to p16.
Highest immunohistochemical expression was observed in Pan-CK which confirmed
the tumours were of epithelial origin and EGFR immunoexpression was
confirmatory for malignancy and degree of metastasis. Neoplasms were confirmed
as SCC by immunoexpression of Pan-CK, EGFR and p53 in malignant tumours
including both well and poorly differentiated SCC of horn.
Author(s) Details:
Vivek Kumar,
Department of Veterinary Pathology, Dau Shri Vasudev Chandrakar
Kamdhenu Vishwavidyalaya, Anjora, Durg- 491001, Chhattisgarh, India.
Dhananjay
Kumar Jolhe,
Department
of Veterinary Pathology, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya,
Anjora, Durg- 491001, Chhattisgarh, India.
Ratan Chandra Ghosh,
Department of Veterinary Pathology, Dau Shri Vasudev Chandrakar
Kamdhenu Vishwavidyalaya, Anjora, Durg- 491001, Chhattisgarh, India.
Rukmani Dewangan,
Department of Veterinary Surgery & Radiology, Dau Shri Vasudev
Chandrakar Kamdhenu Vishwavidyalaya, Anjora, Durg- 491001, Chhattisgarh, India.
Prashant M. Sonkusale,
Department
of Veterinary Pathology, Nagpur Veterinary College, Maharashtra Animal &
Fisheries Science University, Nagpur- 440007, Maharashtra, India.
Sonu
Sharma,
Dr.
Lal Path Labs, Path Vets Veterinary Diagnosis, Chittranjan Park, New
Delhi-110019, India.
Please see the link here: https://stm.bookpi.org/ARBS-V9/article/view/13646
No comments:
Post a Comment