Flowering time is one of the important target traits in a
soybean breeding program. Photoperiod and temperature influence the vegetative
and reproductive development from emergence to maturity in soybean. The
objective of this study was to analyze genetic variation in flowering time
based on agronomic performance and SSR marker. About 108 soybean cultivars were
used for this study. Eight primers relating flowering genes were selected based
on soybean cDNA library from NCBI and TIGR. These primers were designed by
using Primer 3 [1] to amplify fragments of 500-800bp. The optimum melting
temperature was observed for each primer between 58o to 68oC. PCR was followed
by protocol of Crop Genomics Laboratory. PCR products producing a single
discrete band were purified by AccuPre®
PCR purification kit (Bioneer, Korea). The purified PCR product was directly
sequenced using one of the PCR primers with BigDye Terminator Cycle Sequenceing
Kit (Applied Biosystems, Foster City, CA, USA). The labeling reaction mixture
was ethanol-preciputated, and resuspended in 10μl of water. The sequence was
analyzed using ABI 3700 sequencer (Applied Biosystems, Foster City, CA, USA).
In conclusion, there was wide variation in flowering time of soybean. Gene
diversity at nucleotide level will be useful for breeders to make strategy for
soybean improvement in future.
Author(s) Details:
Truong Trong Ngon,
Institute of Food and Biotechnology, Can Tho University, Vietnam.
Suk-Ha Lee,
Crop
Genomics Laboratory, Seoul National University, Republic of Korea.
Please see the link here: https://stm.bookpi.org/RPMAB-V1/article/view/13911
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