Optimizing a Sustainable and Cost-effective DNA Extraction Method from Dry Date Palm Leaves | Chapter 4 | Scientific Research, New Technologies and Applications Vol. 2

Palm date Phoenix dactylifera L. is considered a vital economic fruit tree in Iraq, where its cultivation is of great importance. The extraction of DNA from palm tissues poses significant challenges due to the plant's tough tissue structure and high levels of phenolic and polysaccharide compounds, which hinder DNA isolation efficiently. This study presents an effective method for DNA extraction using Cetyl Trimethyl Ammonium Bromide (CTAB) without the need for liquid nitrogen. A simple and reliable protocol has been developed for isolating DNA from the leaves of six different varieties of palm dates. The resulting DNA showed high concentration and purity, ranging from 567.6 to 1047.1 ng/µL, and 1.82-2.00 nm, respectively. The quality of the extracted DNA was verified using four SSR-PCR markers, which produced 61 clear bands. Our results indicate that this modified CTAB-based method is highly suitable for extracting DNA from palm date tissues without the need for liquid nitrogen, providing a simplified and effective approach for molecular biology applications in date palm research, and this advancement facilitates more accessible and efficient genetic studies of date palms and potentially other plant species with similar extraction challenges.

 

Author (s) Details

Ahmed Yousef Lafta Hzaa
Department of Marine Biology, Marine Science Centre, University of Basrah, Iraq.

 

Aqeel Hadi Abdulwahid Al-Amiry
Department of Horticulture, College of Agricultural, University of Basrah, Iraq.

 

Please see the book here:- https://doi.org/10.9734/bpi/srnta/v2/1994

Finding Genetic Variation in Soybean (Glycine max (L.) Merrill) Flowering Time by Simple Sequence Repeat Marker | Chapter 10 | Research Perspectives of Microbiology and Biotechnology Vol. 1

Flowering time is one of the important target traits in a soybean breeding program. Photoperiod and temperature influence the vegetative and reproductive development from emergence to maturity in soybean. The objective of this study was to analyze genetic variation in flowering time based on agronomic performance and SSR marker. About 108 soybean cultivars were used for this study. Eight primers relating flowering genes were selected based on soybean cDNA library from NCBI and TIGR. These primers were designed by using Primer 3 [1] to amplify fragments of 500-800bp. The optimum melting temperature was observed for each primer between 58o to 68oC. PCR was followed by protocol of Crop Genomics Laboratory. PCR products producing a single discrete band were purified by AccuPre® PCR purification kit (Bioneer, Korea). The purified PCR product was directly sequenced using one of the PCR primers with BigDye Terminator Cycle Sequenceing Kit (Applied Biosystems, Foster City, CA, USA). The labeling reaction mixture was ethanol-preciputated, and resuspended in 10μl of water. The sequence was analyzed using ABI 3700 sequencer (Applied Biosystems, Foster City, CA, USA). In conclusion, there was wide variation in flowering time of soybean. Gene diversity at nucleotide level will be useful for breeders to make strategy for soybean improvement in future.

Author(s) Details:

Truong Trong Ngon,
Institute of Food and Biotechnology, Can Tho University, Vietnam.

Suk-Ha Lee,
Crop Genomics Laboratory, Seoul National University, Republic of Korea.

Please see the link here: https://stm.bookpi.org/RPMAB-V1/article/view/13911