Genome editing has revolutionized molecular biology, with
the Clustered Regularly Interspaced Short Palindromic Repeats and their
associated protein Cas-9 (CRISPR-Cas9) system emerging as a pivotal tool.
Originating from bacterial adaptive immune systems, CRISPR has evolved into a
versatile genome editing mechanism, surpassing previous techniques in
efficiency and accessibility. The CRISPR-Cas-9 system is a highly effective and
precise genome editing tool utilized across various fields. The history of CRISPR
spans decades, from its initial discovery in bacteria to its transformative
applications in mammalian genome editing. The CRISPR-Cas9 system comprises two
essential components: the Cas9 protein and guide RNA (gRNA). Together, they
enable precise DNA cleavage and modification. CRISPR finds applications in
medicine, biotechnology, and beyond, promising personalized treatments for
genetic diseases and advancements in drug development and crop engineering.
Recent advancements include the development of novel Cas proteins and improved
editing techniques, driving the field forward. Efforts to enhance precision and
reliability in gene editing continue to emphasize the ongoing commitment to
unlock the full potential of CRISPR technology. In conclusion, CRISPR-Cas technology
represents a transformative tool in biology, with ongoing efforts focused on
refining its capabilities for precise and reliable gene manipulation. This
paper provides an overview of the history, components, mechanisms,
classifications, applications, advancements, and challenges of CRISPR
technology.
Author(s) Details:
Ankit Sharma,
Department of Anatomy, King George’s Medical University, Lucknow,
India.
Priya Devi,
Department
of Oral Pathology and Microbiology, King George’s Medical University, Lucknow,
India.
Arun Kumar,
Department of Anatomy, King George’s Medical University, Lucknow,
India.
Navneet Kumar,
Department of Anatomy, King George’s Medical University, Lucknow,
India.
Please see the link here: https://stm.bookpi.org/ARBS-V9/article/view/13793
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