This study highlights the production of shatavarins from in
vitro cultures of Asparagus racemosus. Asparagus racemosus is one of the
important medicinal plant found in India, China and other parts of the world.
This plant is known to produce the steroidal saponins the shatavarins. The
major constraint in conventional multiplication method of A. racemosus through seed
propagation is requirement of elaborate pretreatments to break the dormancy and
high mortality of seedlings. Callus cultures of Asparagus racemosus were
initiated in a modified MS medium supplemented with 1.0 mgl-1 NAA and 2,4-D and
0.5 mgl-1 BAP and compared for growth and production of saponin over a period
of 60 days. Saponin production was evaluated at a regular interval of 15 days.
Root calli produces more saponin compared to nodal calli and maximum
accumulation was found to be 10.38 ± 0.14 mg/g of callus after 60 days of
inoculation. Total saponins from the nodal calli were found to be 7.69± 0.136
mg/g of callus. Compared to wild type roots, in vitro cultures showed 20-fold
increase in shatavarin levels. HPLC chromatograms of the cultures indicated
that the overall saponin profile of in vitro and in vivo plant root extract is
similar. Further work needs to done on establishment of suspension cultures,
secretion of saponins into liquid media and evaluation of bioactivities to
validate the protocol for scale up studies.
Author(s) Details:
Mashitha Pise,
Department of Biochemistry, Hislop College, Temple Road, Civil Lines, Nagpur, Maharashtra – 440001, India.
Avinash Upadhyay,
Department of Biochemistry, Hislop College, Temple Road, Civil Lines, Nagpur, Maharashtra – 440001, India.
Please see the link here: https://stm.bookpi.org/ACPR-V6/article/view/13413
No comments:
Post a Comment