G-Quadruplex and Ligand Interactions | Chapter 23 | Socio-Scientific Interaction in Diabetes and Cancer and Its Management

 Designing ligands to interact with G-quadruplex (G4) of DNA is challenging due to the complexity of G4 structures and the lack of efficient experimental methods to characterize G4/ligand interactions. Existing methods can be categorized into structure-based (e.g., CD, NMR, X-ray crystallography), affinity-based (e.g., SPR, ITC, MS), and high-throughput (e.g., FRET, G4-FID, affinity chromatography, microarrays) approaches, each with its own advantages and disadvantages. High-throughput methods are emerging as a promising alternative to traditional methods for screening and designing new G4 ligands, as they are faster and more cost-effective.

G4s are found in both DNA and RNA and play an important role in a variety of physiological processes, including cancer and neurological disorders. Targeting G4s with ligands is a promising therapeutic strategy for these diseases. A significant number of G4 ligands have been developed and investigated, and most of them are deposited in the G4 Ligands Database 2.1 (http://www.g4ldb.com/).

Author(s) Details:

Patnaik Amit,
Department of Biotechnology, NIST Institute of Science and Technology Autonomous, Institute Park, Pallur Hills, Berhampur, Odisha – 761008, India.

Sinha Anita,
Biotechnology, University Department of Botany, Ranchi University Ranchi, Jharkhand-834008, India.

Oraon Vinay,
Biotechnology, University Department of Botany, Ranchi University Ranchi, Jharkhand-834008, India.

Sahu Duryodhan,
Department of Chemistry, NIST Institute of Science and Technology Autonomous, Institute Park, Pallur Hills, Berhampur, Odisha – 761008, India.

Please see the link here: https://stm.bookpi.org/SSIDCIM/article/view/12741