Sensitivity of Rapid Diagnostic Test Compared to qRT-PCR in the COVID-19 Management Strategy in Tunisia | Chapter 9 | Advanced Concepts in Medicine and Medical Research Vol. 8

 The objective concerning this chapter isto investigatethe benefit of the swift antigenic detection of SARS-CoV-2 and to demonstrate the gift of this method compared to real-opportunity RT-PCR.In this study, the SARS-CoV-2 nucleocapsid N irritant rapid diagnostic test (Standard Covid-19 Ag Test, SD Biosensor) was acted. The sample size of the study was49 subjects. Real-occasion RT-PCR testing was performed only in 12 cases. Extraction provisions (Model: VDR-B096V) was used for the extraction of deoxyribonucleic acid from various container types. The extracted nucleic acids are appropriate to PCR, real-occasion PCR, enzymatic backlashes and others.Nasopharayngeal swabs were taken from cases whose mean age was 35 years (range 23-68 age) and who presented individual of the following symptoms: dry cough (30.61%), breast tightness (28%), fever (28%), problem (24.48%), asthenia (22.44%) and diarrhea in only 14.28%. Concurrently with an activity the onset of syndromes and the completion of the test ranged from 0 to 2 days. In the preferred sample14 (28.57%) were positive and 35 (71.42%) were negative for all fast tests performed. Of the samples tested, 44 arose different IMKO areas. RT-PCR was performed in 8 patients whose fast tests were negative and gave a certain result in 2 cases. Seeing the significance of the global community health emergency all along the pandemic, it was necessary to complete activity large studies to better comprehend the issue of potential SARS-CoV-2 recurrence in COVID-19 sufferers.Due to the limited rate of patients accompanying prolonged SARS-Cov-2 RNA adaptation and the high fake negative rate of viral test results, recurrence of helpful SARS-Cov-2 may knowledge from false negative RT-PCR results.SARS-Cov-2 revival or re-infection will be a persistent and bothering problem.It has existed observed that the detection of SARS-CoV-2 needs expected evaluated and distinguished to the standard RT-PCR technique, which frequently offers significantly better subtlety.

Author(s) Details:

Hajer Kilani,
Faculty of Medicine, LR99ES09 Laboratory of Antibiotic Resistance, University of Tunis El Manar, Tunis, Tunisia.

Please see the link here: https://stm.bookpi.org/ACMMR-V8/article/view/12791