Using quantitative polymerase chain reaction and traditional polymerase chain reaction (PCR), we sought to describe the sex differentiation expression of the Sox9 and Foxl2 genes and polymorphisms, respectively. To investigate the connection between regulatory region polymorphisms and Sox9 and Foxl2 gene expression during the developmental stage, we examined the coding regions of these genes in two Nile tilapia (Oreochromis niloticus) strains exposed to various temperatures. 64 mice were used in the experiment six days after conception (dpf). Animal tissue samples were frequently taken at intervals of 7 days, from day 0 to day 28. Between treatments and strains, there were differences in Sox9 and Foxl2 gene expression that were statistically significant (P 0.001). Sox9 in the Supreme strain increased moderately (*P 0.05) from 12 to 19 dpf. At 19 dpf, this strain had a 1.5–2 fold increase in Foxl2 overexpression (P 0.001). At 19 dpf, the temperature (35°C) significantly boosted Foxl2 and Sox9 expression. Regions with polymorphism were only seen in Sox9. In neither Oreochromis niloticus strain did temperature significantly alter Sox9 expression. Furthermore, the expression regulation of other genes involved in sex determination did not directly correlate with it. As a result, we were unable to find a connection between Oreochromis niloticus gene expression and sex reversal by temperature.
Author (s) Details
Daralyns Borges Macedo
Applied Genetics Laboratory,
Socio-Environmental and Water Resources Institute, Amazon Federal Rural
University, Belém, PA, Brazil.
Natalia Bianca Caires Medeiros
Applied Genetics Laboratory,
Socio-Environmental and Water Resources Institute, Amazon Federal Rural
University, Belém, PA, Brazil.
Cristina Helena Maria Moreira Vernetti
Animal Genetic Engineering Laboratory,
Biology Institute, Ecology, Zoology and Genetics Department, Pelotas Federal
University, Pelotas, RS, Brazil.
Marcela Cristina Flexa do Amaral
Applied Genetics Laboratory,
Socio-Environmental and Water Resources Institute, Amazon Federal Rural
University, Belém, PA, Brazil.
Carla Giovane Ávila Moreira
Animal Genetic Engineering Laboratory,
Biology Institute, Ecology, Zoology and Genetics Department, Pelotas Federal
University, Pelotas, RS, Brazil.
Debora Sayumi Doami Melo
Applied Genetics Laboratory,
Socio-Environmental and Water Resources Institute, Amazon Federal Rural
University, Belém, PA, Brazil.
Heden Luiz Marques Moreira
Animal Genetic Engineering Laboratory,
Biology Institute, Ecology, Zoology and Genetics Department, Pelotas Federal
University, Pelotas, RS, Brazil.
Marília Danyelle Nunes Rodrigues
Applied Genetics Laboratory,
Socio-Environmental and Water Resources Institute, Amazon Federal Rural
University, Belém, PA, Brazil.
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