Could Methanol Leaf Extracts of Erythrina variegate L. be a Potential Source of Anticancer Molecule against Breast Cancer Cell Lines? – A Preliminary in vitro Investigation | Chapter 3 | Current Aspects in Pharmaceutical Research and Development Vol. 1

Traditional/folklore medicine in India is reported to use Erythrina variegata L. (E. variegata) species in the treatment of cancer. As a result, the anticancer potential of this plant's leaves might be investigated. The goals of this study were to: I assess the anticancer potential of crude extracts of E. variegata leaves using two solvents with different polarities, methanol and chloroform, ii) investigate the mechanism of cytotoxicity using the most effective extract, and iii) identify the phytochemicals linked to the cytotoxicity of the most active extract. Methods: The cytotoxicity of E. variegata methanol soluble (EVM) and chloroform soluble (EVC) extracts was determined using the [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide] MTT test using MCF-7 and MDA-MB-231 breast cancer cell lines. The nucleoprotein concentration and cell shape of EVM (more effective) treated MDA-MB-231 cells were also investigated. High-Resolution Liquid Chromatography Mass Spectrometry was used to determine the phytochemical makeup of EVM (HRLCMS). Results: On MCF-7 and MDA-MB-231 cells, EVM was the most efficient extract, with an IC50 of 92 g/mL and 143 g/mL, respectively. The EVM (IC50 143 g/mL) treated MDA-MB-231 had a nucleoprotein concentration of 58.2 percent and an apoptotic index of 51.8 percent. MDA-MB-231 cells treated with EVM displayed morphological alterations that were suggestive of apoptosis. HRLCMS discovered phytochemicals that are known to be cytotoxic, such as rutin, podocarpatriene, and cepharanthine. Conclusion: The results of this investigation imply that methanol extracts of E. variegata leaves could be a source of anticancer compounds.



Author(s) Details

Dr. Vaishali Rai M
Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, India and Department of Microbiology, St Aloysius College (Autonomous), Mangalore, India.

Vinitha Ramanath Pai
Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, India.

Mr. Samuel Kevin
Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, India.


Herga P. Kedilaya
Department of Biochemistry, Srinivas Institute of Medical Sciences, Mangalore, India.


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