Bacillus anthracis could be utilised as a biological weapon or as a kind of bioterorism in the future. Attacks by Bacillus anthracis are extremely fatal, and spore transfer is simple and cheap. The purpose of this study was to find spores of Bacillus anthracis. Bacillus anthracis isolates were grown on serum agar and then sheep blood medium to induce capsule formation. Schaefer and Fultton's approach was used to paint the spores that had developed. Precipitation and immuno-chromatography techniques were used to detect Bacillus anthracis spores. Bacillus anthracis isolates were grown on serum agar before being transferred to sheep blood medium to induce capsule formation. Schaefer and Fultton's approach was used to paint the produced spores. Precipitation and immuno-chromatography techniques were used to identify Bacillus anthracis spores. As seen by painting with the Schaeffer and Fulton method, Bacillus anthracis spores are green. A precipitation interaction between Bacillus anthracis spores and goat antibody anti anthrosa resulted in a silver white hue. Immunochromatography was employed to identify Anthrosa of Bacillus anthracis spores using anti-anthrosa goat antibody. Anthrosa has a molecular weight of 148 kDa. Conclusions: Bacillus anthracis spores can be detected using precipitation and immuno-chromatography using anti-anthrosa goat antibody. Antibodies to Bacillus anthracis spores have been observed to react well with anti-anthrosa antibodies from goats.
Author (S) Details
Edy Parwanto
Department of Biology, Faculty of Medicine, Universitas Trisakti, Jakarta, Indonesia.
Alfred Pakpahan
Department of Biology, Faculty of Medicine, Universitas Trisakti, Jakarta, Indonesia.
Hosea Jaya Edy
Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Sam Ratulangi, Manado, Indonesia.
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