In biological wastewater treatment systems, high lipid (fats and oils) concentrations induce clogged drain pipes and limit the action of bacteria. The potential of lipid-degrading bacteria to degrade lipids was examined for use in the treatment of lipid-contaminated wastewater. On LB medium, 43 vegetable oil-contaminated wastewater samples from several food processing companies and restaurants in five districts of CanTho city, Vietnam yielded 102 bacterial isolates. On Tw20 medium, 61 of the isolates produced clear zones. 11 of the 61 bacterial isolates generated substantial halos, indicating that they had the capability to degrade vegetable oil in contaminated wastewater. PCR and DNA sequencing were used to identify these 11 baterial isolates. BLAT N software was used to compare the DNA sequencing findings to the NCBI's GenBank database. The DNA sequences of a few isolates were found to be very similar to the GenBank references (between 97 percent and 99 percent ). Bacilli were found in two isolates (18.18 percent) while Gammaproteobacteria were found in nine isolates (81.82 percent ). Gammaproteobacteria had the greatest Theta values based on Pi (nucleotide diversity). For each group, theta value (per sequence) from S of SNP for DNA polymorphism was computed, and the 11 lipid-degrading strains possessed a lot of genetic variety. Because of its strong ability to degrade lipids and biosafety, the results suggested Acinetobacteria soli strain AL3 as a promising bioproduct for wastewater treatment.
Author (S) Details
Ngo Thanh Phong
College of Natural Sciences, Can Tho University, Vietnam.
Bui The Vinh
CanTho Dairy Factory, Vietnam.
Cao Ngoc Diep
Biotechnology R&D Institute, Can Tho University, Vietnam.
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