The present chapter described a fast, simple, and
small-scale method of sample preparation followed by HPLC coupled with a
photodiode array detector (PDAD) for quantification of canthaxanthin in chicken
liver and fat, egg yolk. The permitted Canthaxanthin (CX) is a red pigment and
is commonly added to feeds for chickens in order to achieve the desired chicken
product color. The HPLC-PDAD was performed on a C18 column with an isocratic
mobile phase. The analyte was extracted from the sample using a handheld ultrasonic
homogenizer and purified by MonoSpin®-SI, a centrifugal monolithic silica spin
mini-columns, and quantified < 15 min. The system-suitability evaluation is
an essential parameter of HPLC determination, and it ascertains the strictness
of the system used. The suitability was evaluated as the relative standard
deviations of peak areas and retention times calculated for 20 replicate
injections of a spiked egg yolk sample. The proposed method obtained average
recoveries for the analyte analyte in the range of 93.5 - 101.0% with relative
standard deviations ≤ 2.7%. The Limits of quantification in the liver, fat, and
egg yolk were 0.48, 0.47, and 0.5 µg/g, respectively. The present procedure
provided an easy-to-use, rapid, and space-saving and resulted in high recovery
and repeatability with considerable saving of analysis time/cost. The procedure
may be proposed as an international harmonized method for determining CX in
domestic/imported chicken products.
Author(s) Details:
Naoto Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan
University, Osaka 558-8585, Japan.
Please see the link here: https://stm.bookpi.org/IBS-V3/article/view/14331
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