A Simple, Robust and Handheld Sample Preparation Technique for Quantifying Canthaxanthin in Chicken Liver, Fat and Egg Yolk | Chapter 13 | Innovations in Biological Science Vol. 3

The present chapter described a fast, simple, and small-scale method of sample preparation followed by HPLC coupled with a photodiode array detector (PDAD) for quantification of canthaxanthin in chicken liver and fat, egg yolk. The permitted Canthaxanthin (CX) is a red pigment and is commonly added to feeds for chickens in order to achieve the desired chicken product color. The HPLC-PDAD was performed on a C18 column with an isocratic mobile phase. The analyte was extracted from the sample using a handheld ultrasonic homogenizer and purified by MonoSpin®-SI, a centrifugal monolithic silica spin mini-columns, and quantified < 15 min. The system-suitability evaluation is an essential parameter of HPLC determination, and it ascertains the strictness of the system used. The suitability was evaluated as the relative standard deviations of peak areas and retention times calculated for 20 replicate injections of a spiked egg yolk sample. The proposed method obtained average recoveries for the analyte analyte in the range of 93.5 - 101.0% with relative standard deviations ≤ 2.7%. The Limits of quantification in the liver, fat, and egg yolk were 0.48, 0.47, and 0.5 µg/g, respectively. The present procedure provided an easy-to-use, rapid, and space-saving and resulted in high recovery and repeatability with considerable saving of analysis time/cost. The procedure may be proposed as an international harmonized method for determining CX in domestic/imported chicken products.

Author(s) Details:

Naoto Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka 558-8585, Japan.

Please see the link here: https://stm.bookpi.org/IBS-V3/article/view/14331

Space-saving Sample Preparation Procedure to Monitor Residual Canthaxanthin in Hen Egg Yolk | Chapter 04 | Emerging Challenges in Agriculture and Food Science Vol. 4

 This chapter offers an appropriate analytical approach for routine antimicrobial drug residue monitoring in food. Canthaxanthin (CX), a colouring ingredient, was quantified in hen egg yolk using a simpler and space-saving sample preparation procedure followed by an isocratic mobile phase high-performance liquid chromatography (HPLC) connected diode array detector. Using a handheld ultrasonic homogenizer and 0.6 ml solvent, the analyte was extracted from the sample and purified using a normal-phase InertSep® CN mini-syringe column. For the analysis of a single sample, the total time and solvent consumption were 1 hour and 7 ml, respectively. Linearity, accuracy, precision, and system suitability are all well within the worldwide method acceptance standards. The process could be presented as a standardised analytical method for monitoring CX residues in hen egg yolk on a regular basis.

Author(S) Details

Naoto Furusawa
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka 558-8585, Japan.

View Book:- https://stm.bookpi.org/ECAFS-V4/article/view/6403

Small-Scale and Time Shortening Sample Preparation Procedure to Quantify Astaxanthin, Canthaxanthin, and β-Apo-8’-Carotenoic Acid Ethyl Ester in Hen’s Egg Yolk | Chapter 6 | New Innovations in Chemistry and Biochemistry Vol.8

 Because eggs are highly nutritious, inexpensive, and readily available, they are a very important and basic food. It is critical to improve residual monitoring of eggs in order to maintain their safety. The current chapter described a simple, small-scale, and time-saving sample preparation procedure followed by simultaneous quantification of astaxanthin (AX), canthaxanthin (CX), and (beta)-apo-8'-carotenoic acid ethyl ester (ACAEE) in hen's egg yolk using a high-performance liquid chromatography (HPLC) coupled photodiode array (PDA) detector. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analytes were extracted from the sample with a handheld ultrasonic homogenizer in less than 30 minutes, purified with MonoSpin®-SI, a centrifugal monolithic silica spin mini-column, and quantified. For the three analytes, the proposed method provided average recoveries of 70.5-101.1 percent, with relative standard deviations of (le)5.0 percent. The quantitation limits for AX, CX, and ACAEE were 0.3 (mu)g g-1, 0.5 (mu)g g-1, and 1.0 (mu)g g-1, respectively. The examination of a single sample took about 30 minutes in total.

Author(s) Details:

N. Furusawa,
Graduate School of Human Life Science, Osaka Metropolitan University, Osaka, 558-8585, Japan.

Please see the link here: https://stm.bookpi.org/NICB-V8/article/view/6285